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Islet oxygen consumption rate dose predicts insulin independence for first clinical islet allotransplants.
Kitzmann, J P; O'Gorman, D; Kin, T; Gruessner, A C; Senior, P; Imes, S; Gruessner, R W; Shapiro, A M J; Papas, K K.
Afiliación
  • Kitzmann JP; Department of Surgery, University of Arizona, Tucson, AZ.
  • O'Gorman D; Clinical Islet Transplant Program, University of Alberta, Edmonton, Alberta, Canada.
  • Kin T; Clinical Islet Transplant Program, University of Alberta, Edmonton, Alberta, Canada.
  • Gruessner AC; Department of Surgery, University of Arizona, Tucson, AZ.
  • Senior P; Clinical Islet Transplant Program, University of Alberta, Edmonton, Alberta, Canada.
  • Imes S; Clinical Islet Transplant Program, University of Alberta, Edmonton, Alberta, Canada.
  • Gruessner RW; Department of Surgery, University of Arizona, Tucson, AZ.
  • Shapiro AM; Clinical Islet Transplant Program, University of Alberta, Edmonton, Alberta, Canada.
  • Papas KK; Department of Surgery, University of Arizona, Tucson, AZ. Electronic address: kkpapas@surgery.arizona.edu.
Transplant Proc ; 46(6): 1985-8, 2014.
Article en En | MEDLINE | ID: mdl-25131089
BACKGROUND: Human islet allotransplantation for the treatment of type 1 diabetes is in phase III clinical trials in the U.S. and is the standard of care in several other countries. Current islet product release criteria include viability based on cell membrane integrity stains, glucose-stimulated insulin release, and islet equivalent (IE) dose based on counts. However, only a fraction of patients transplanted with islets that meet or exceed these release criteria become insulin independent following 1 transplant. Measurements of islet oxygen consumption rate (OCR) have been reported as highly predictive of transplant outcome in many models. METHOD: In this article we report on the assessment of clinical islet allograft preparations using OCR dose (or viable IE dose) and current product release assays in a series of 13 first transplant recipients. The predictive capability of each assay was examined and successful graft function was defined as 100% insulin independence within 45 days post-transplant. RESULTS: OCR dose was most predictive of CTO. IE dose was also highly predictive, while glucoses stimulated insulin release and membrane integrity stains were not. CONCLUSION: OCR dose can predict CTO with high specificity and sensitivity and is a useful tool for evaluating islet preparations prior to clinical human islet allotransplantation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Consumo de Oxígeno / Trasplante de Islotes Pancreáticos / Islotes Pancreáticos / Diabetes Mellitus Tipo 1 Tipo de estudio: Etiology_studies / Incidence_studies / Observational_studies / Prognostic_studies / Risk_factors_studies Límite: Humans Idioma: En Revista: Transplant Proc Año: 2014 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Consumo de Oxígeno / Trasplante de Islotes Pancreáticos / Islotes Pancreáticos / Diabetes Mellitus Tipo 1 Tipo de estudio: Etiology_studies / Incidence_studies / Observational_studies / Prognostic_studies / Risk_factors_studies Límite: Humans Idioma: En Revista: Transplant Proc Año: 2014 Tipo del documento: Article Pais de publicación: Estados Unidos