Construction and characterization of a repetitive DNA library in Parodontidae (Atinopterygii:Characiformes): a genomic and evolutionary approach to the degeneration of the w sex cromosome.

Schemberger, Michelle Orane; Oliveira, Jordana Inácio Nascimento; Nogaroto, Viviane; Almeida, Mara Cristina; Artoni, Roberto Ferreira; Cestari, Marta Margarete; Moreira-Filho, Orlando; Vicari, Marcelo Ricardo

Schemberger, Michelle Orane; Oliveira, Jordana Inácio Nascimento; Nogaroto, Viviane; Almeida, Mara Cristina; Artoni, Roberto Ferreira; Cestari, Marta Margarete; Moreira-Filho, Orlando; Vicari, Marcelo Ricardo.

Afiliación

Schemberger MO; 1 Programa de Pós-Graduação em Genética, Departamento de Genética, Centro Politécnico, Universidade Federal do Paraná , Curitiba, Paraná State, Brazil .

Zebrafish ; 11(6): 518-27, 2014 Dec.

Article en En | MEDLINE | ID: mdl-25122415

RESUMEN

Repetitive DNA sequences, including tandem and dispersed repeats, comprise a large portion of eukaryotic genomes and are important for gene regulation, sex chromosome differentiation, and karyotype evolution. In Parodontidae, only the repetitive DNAs WAp and pPh2004 and rDNAs were previously studied using fluorescence in situ hybridization. This study aimed to build a library of repetitive DNA in Parodontidae. We isolated 40 clones using Cot-1; 17 of these clones exhibited similarity to repetitive DNA sequences, including satellites, minisatellites, microsatellites, and class I and class II transposable elements (TEs), from Danio rerio and other organisms. The physical mapping of the clones to chromosomes revealed the presence of a satellite DNA, a Helitron element, and degenerate short interspersed element (SINE), long interspersed element (LINE), and tc1-mariner elements on the sex chromosomes. Some clones exhibited dispersed signals; other sequences were not detected. The 5S rDNA was detected on an autosomal pair. These elements likely function in the molecular degeneration of the W chromosome in Parodontidae. Thus, the location of these elements on the chromosomes is important for understanding the function of these repetitive DNAs and for integrative studies with genome sequencing. The presented data demonstrate that an intensive invasion of TEs occurred during W sex chromosome differentiation in the Parodontidae.

Asunto(s)

Characiformes/genética; Evolución Molecular; Biblioteca de Genes; Secuencias Repetitivas de Ácidos Nucleicos/genética; Cromosomas Sexuales/genética; Animales; Secuencia de Bases; Hibridación Fluorescente in Situ; Datos de Secuencia Molecular; Mapeo Físico de Cromosoma; ARN Ribosómico 5S/genética; Análisis de Secuencia de ADN; Especificidad de la Especie

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cromosomas Sexuales / Secuencias Repetitivas de Ácidos Nucleicos / Biblioteca de Genes / Evolución Molecular / Characiformes Límite: Animals Idioma: En Revista: Zebrafish Asunto de la revista: BIOLOGIA Año: 2014 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Estados Unidos

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