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Low birth weight is associated with adiposity, impaired skeletal muscle energetics and weight loss resistance in mice.
Beauchamp, B; Ghosh, S; Dysart, M W; Kanaan, G N; Chu, A; Blais, A; Rajamanickam, K; Tsai, E C; Patti, M-E; Harper, M-E.
Afiliación
  • Beauchamp B; Department of Biochemistry, Microbiology, and Immunology, Faculty of Medicine, University of Ottawa, Ottawa, Ontario, Canada.
  • Ghosh S; Cardiovascular & Metabolic Disorders Program, Duke-NUS Graduate Medical School, Singapore, Singapore.
  • Dysart MW; Department of Biochemistry, Microbiology, and Immunology, Faculty of Medicine, University of Ottawa, Ottawa, Ontario, Canada.
  • Kanaan GN; Department of Biochemistry, Microbiology, and Immunology, Faculty of Medicine, University of Ottawa, Ottawa, Ontario, Canada.
  • Chu A; 1] Department of Biochemistry, Microbiology, and Immunology, Faculty of Medicine, University of Ottawa, Ottawa, Ontario, Canada [2] Ottawa Institute of Systems Biology, University of Ottawa, Ottawa, Ontario, Canada.
  • Blais A; 1] Department of Biochemistry, Microbiology, and Immunology, Faculty of Medicine, University of Ottawa, Ottawa, Ontario, Canada [2] Ottawa Institute of Systems Biology, University of Ottawa, Ottawa, Ontario, Canada.
  • Rajamanickam K; Division of Neurosurgery, Department of Surgery, Faculty of Medicine, Ottawa Hospital Research Institute, University of Ottawa, Ottawa, Ontario, Canada.
  • Tsai EC; Division of Neurosurgery, Department of Surgery, Faculty of Medicine, Ottawa Hospital Research Institute, University of Ottawa, Ottawa, Ontario, Canada.
  • Patti ME; Division of Integrative Physiology and Metabolism, Joslin Diabetes Center, Boston, MA, USA.
  • Harper ME; 1] Department of Biochemistry, Microbiology, and Immunology, Faculty of Medicine, University of Ottawa, Ottawa, Ontario, Canada [2] Ottawa Institute of Systems Biology, University of Ottawa, Ottawa, Ontario, Canada.
Int J Obes (Lond) ; 39(4): 702-11, 2015 Apr.
Article en En | MEDLINE | ID: mdl-25091727
BACKGROUND: In utero undernutrition is associated with obesity and insulin resistance, although its effects on skeletal muscle remain poorly defined. Therefore, in the current study we explored the effects of in utero food restriction on muscle energy metabolism in mice. METHODS: We used an experimental mouse model system of maternal undernutrition during late pregnancy to examine offspring from undernourished dams (U) and control offspring from ad libitum-fed dams (C). Weight loss of 10-week-old offspring on a 4-week 40% calorie-restricted diet was also followed. Experimental approaches included bioenergetic analyses in isolated mitochondria, intact (permeabilized) muscle and at the whole body level. RESULTS: U have increased adiposity and decreased glucose tolerance compared to C. Strikingly, when U are put on a 40% calorie-restricted diet they lose half as much weight as calorie-restricted controls. Mitochondria from muscle overall from U had decreased coupled (state 3) and uncoupled (state 4) respiration and increased maximal respiration compared to C. Mitochondrial yield was lower in U than C. In permeabilized fiber preparations from mixed fiber-type muscle, U had decreased mitochondrial content and decreased adenylate-free leak respiration, fatty acid oxidative capacity and state 3 respiratory capacity through complex I. Fiber maximal oxidative phosphorylation capacity did not differ between U and C but was decreased with calorie restriction. CONCLUSIONS: Our results reveal that in utero undernutrition alters metabolic physiology through a profound effect on skeletal muscle energetics and blunts response to a hypocaloric diet in adulthood. We propose that mitochondrial dysfunction links undernutrition in utero with metabolic disease in adulthood.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Glucemia / Recién Nacido de Bajo Peso / Músculo Esquelético / Restricción Calórica / Desnutrición Tipo de estudio: Risk_factors_studies Aspecto: Determinantes_sociais_saude Límite: Animals Idioma: En Revista: Int J Obes (Lond) Asunto de la revista: METABOLISMO Año: 2015 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Reino Unido
Texto completo
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Glucemia / Recién Nacido de Bajo Peso / Músculo Esquelético / Restricción Calórica / Desnutrición Tipo de estudio: Risk_factors_studies Aspecto: Determinantes_sociais_saude Límite: Animals Idioma: En Revista: Int J Obes (Lond) Asunto de la revista: METABOLISMO Año: 2015 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Reino Unido