Your browser doesn't support javascript.
loading
blaCTX-M-15-carrying Escherichia coli and Salmonella isolates from livestock and food in Germany.
Fischer, Jennie; Rodríguez, Irene; Baumann, Beatrice; Guiral, Elisabeth; Beutin, Lothar; Schroeter, Andreas; Kaesbohrer, Annemarie; Pfeifer, Yvonne; Helmuth, Reiner; Guerra, Beatriz.
Afiliación
  • Fischer J; Federal Institute for Risk Assessment, National Reference Laboratory for Antimicrobial Resistance, Department Biological Safety, Max-Dohrn Straße 8-10, D-10589 Berlin, Germany.
  • Rodríguez I; Federal Institute for Risk Assessment, National Reference Laboratory for Antimicrobial Resistance, Department Biological Safety, Max-Dohrn Straße 8-10, D-10589 Berlin, Germany Hospital Universitario Ramón y Cajal, Servicio de Microbiología, Madrid, Spain.
  • Baumann B; Federal Institute for Risk Assessment, National Reference Laboratory for Antimicrobial Resistance, Department Biological Safety, Max-Dohrn Straße 8-10, D-10589 Berlin, Germany.
  • Guiral E; Federal Institute for Risk Assessment, National Reference Laboratory for Antimicrobial Resistance, Department Biological Safety, Max-Dohrn Straße 8-10, D-10589 Berlin, Germany Barcelona Centre for International Health Research (CRESIB), Hospital Clínic-Universidad de Barcelona, Barcelona, Spain.
  • Beutin L; Federal Institute for Risk Assessment, National Reference Laboratory for Antimicrobial Resistance, Department Biological Safety, Max-Dohrn Straße 8-10, D-10589 Berlin, Germany.
  • Schroeter A; Federal Institute for Risk Assessment, National Reference Laboratory for Antimicrobial Resistance, Department Biological Safety, Max-Dohrn Straße 8-10, D-10589 Berlin, Germany.
  • Kaesbohrer A; Federal Institute for Risk Assessment, National Reference Laboratory for Antimicrobial Resistance, Department Biological Safety, Max-Dohrn Straße 8-10, D-10589 Berlin, Germany.
  • Pfeifer Y; Robert Koch Institute, FG13 Nosocomial Pathogens and Antimicrobial Resistance, Wernigerode, Germany.
  • Helmuth R; Federal Institute for Risk Assessment, National Reference Laboratory for Antimicrobial Resistance, Department Biological Safety, Max-Dohrn Straße 8-10, D-10589 Berlin, Germany.
  • Guerra B; Federal Institute for Risk Assessment, National Reference Laboratory for Antimicrobial Resistance, Department Biological Safety, Max-Dohrn Straße 8-10, D-10589 Berlin, Germany beatriz.guerra@bfr.bund.de.
J Antimicrob Chemother ; 69(11): 2951-8, 2014 Nov.
Article en En | MEDLINE | ID: mdl-25074857
OBJECTIVES: The characterization of CTX-M-15 ß-lactamase-producing Escherichia coli and Salmonella isolates originating mainly from German livestock and food. METHODS: E. coli (526, mainly commensals) and Salmonella (151) non-human isolates resistant to third-generation cephalosporins, originating from routine and monitoring submissions (2003-12) to the Federal Institute for Risk Assessment and different national targeted studies (2011-12), were examined for the presence of blaCTX-M-15 genes by PCR amplification/sequencing. Additional resistance and virulence genes were screened by DNA microarray and PCR amplification. E. coli isolates with blaCTX-M-15 were characterized by phylogenetic grouping, PFGE and multilocus sequence typing (MLST). The blaCTX-M-15 plasmids were analysed by replicon typing, plasmid MLST, S1 nuclease PFGE and Southern blot hybridization experiments. RESULTS: Twenty-one E. coli (livestock, food and a toy; 4.0%) and two Salmonella (horse and swine; 1.3%) isolates were CTX-M-15 producers. E. coli isolates were mainly ascribed to three clonal lineages of sequence types ST678 (German outbreak with enteroaggregative Shiga-toxin-producing E. coli O104:H4; salmon, cucumber and a toy), ST410 (poultry, swine and cattle farms) and ST167/617 (swine farms and turkey meat). The blaCTX-M-15 genes were located on IncI1 and multireplicon IncF plasmids or on the chromosome of E. coli ST410 isolates. CONCLUSIONS: The prevalence of CTX-M-15-producing isolates from non-human sources in Germany is still low. The blaCTX-M-15 gene is, however, present in multidrug-resistant E. coli clones with pathogenic potential in livestock and food. The maintenance of the blaCTX-M-15 gene due to chromosomal carriage is noteworthy. The possibility of an exchange of CTX-M-15-producing isolates or plasmids between livestock and humans (in both directions) deserves continuous surveillance.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Salmonella / Beta-Lactamasas / Escherichia coli / Ganado / Abastecimiento de Alimentos Tipo de estudio: Risk_factors_studies Aspecto: Determinantes_sociais_saude Límite: Animals / Humans País/Región como asunto: Europa Idioma: En Revista: J Antimicrob Chemother Año: 2014 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Salmonella / Beta-Lactamasas / Escherichia coli / Ganado / Abastecimiento de Alimentos Tipo de estudio: Risk_factors_studies Aspecto: Determinantes_sociais_saude Límite: Animals / Humans País/Región como asunto: Europa Idioma: En Revista: J Antimicrob Chemother Año: 2014 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Reino Unido