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Aberrant frequency of IL-10-producing B cells and its association with Treg/Th17 in adult primary immune thrombocytopenia patients.
Hua, Fanli; Ji, Lili; Zhan, Yanxia; Li, Feng; Zou, Shanhua; Chen, Lihang; Gao, Song; Li, Ying; Chen, Hao; Cheng, Yunfeng.
Afiliación
  • Hua F; Department of Haematology, Zhongshan Hospital, Fudan University, 180 Fenglin Road, Shanghai 200032, China ; Department of Haematology, Jinshan Hospital, Fudan University, Shanghai 201508, China.
  • Ji L; Department of Haematology, Zhongshan Hospital, Fudan University, 180 Fenglin Road, Shanghai 200032, China.
  • Zhan Y; Department of Haematology, Zhongshan Hospital, Fudan University, 180 Fenglin Road, Shanghai 200032, China.
  • Li F; Department of Haematology, Zhongshan Hospital, Fudan University, 180 Fenglin Road, Shanghai 200032, China.
  • Zou S; Department of Haematology, Zhongshan Hospital, Fudan University, 180 Fenglin Road, Shanghai 200032, China.
  • Chen L; Department of Haematology, Zhongshan Hospital, Fudan University, 180 Fenglin Road, Shanghai 200032, China ; Department of Internal Medicine, John A. Burns School of Medicine, University of Hawaii, HI 96813, USA.
  • Gao S; Department of Haematology, Jinshan Hospital, Fudan University, Shanghai 201508, China.
  • Li Y; Department of Haematology, Jinshan Hospital, Fudan University, Shanghai 201508, China.
  • Chen H; Department of Cardiothoracic Surgery, Tongji Hospital, Tongji University, Shanghai 200065, China.
  • Cheng Y; Department of Haematology, Zhongshan Hospital, Fudan University, 180 Fenglin Road, Shanghai 200032, China ; Research Centre, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
Biomed Res Int ; 2014: 571302, 2014.
Article en En | MEDLINE | ID: mdl-25057496
BACKGROUND: Regulatory B cells (Breg) are a distinct B cell subset with immunoregulatory properties. Pivotal to Breg function is interleukin-10. This study was to investigate the role of IL-10-producing B cell (B10) and its association with Treg and Th17 subsets in immune thrombocytopenia (ITP) patients. METHODS: Peripheral blood mononuclear cells from ITP patients and controls were stimulated with PMA, ionomycin, and Brefeldin A. The frequencies of CD19(+)IL-10(+) B cells, CD3(+)CD4(+)IL-17(+) Th17 cells, and CD4(+)CD25(hi)Foxp3(+) Treg cells were analyzed by flow cytometry. The mRNA expression of Foxp3 and RORγt was detected by real-time quantitative PCR. RESULTS: The number of B10 cells was elevated in ITP patients. After first-line therapies, it remained at high level in patients who achieved complete or partial response but decreased in those who acquired no response. There was a positive correlation between B10 cells and Tregs in ITP both before and after therapies. The ratio of Treg/Th17 decreased in ITP, and it strongly correlated with B10 cells. CONCLUSIONS: The frequency of B10 cells is elevated in ITP and it correlates with both the Tregs counts and the Treg/Th17 ratio. B10 cells to regulate functional T cell subsets might be impaired in patients with ITP.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Linfocitos B / Púrpura Trombocitopénica Idiopática / Interleucina-10 / Células Th17 Tipo de estudio: Risk_factors_studies Límite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Biomed Res Int Año: 2014 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Linfocitos B / Púrpura Trombocitopénica Idiopática / Interleucina-10 / Células Th17 Tipo de estudio: Risk_factors_studies Límite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Biomed Res Int Año: 2014 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos