Characterization and phylogenetic analysis of a highly pathogenic avian influenza H5N1 virus isolated from diseased ostriches (Struthio camelus) in the Kingdom of Saudi Arabia.
Avian Dis
; 58(2): 309-12, 2014 Jun.
Article
en En
| MEDLINE
| ID: mdl-25055639
During 2007, two outbreaks of avian influenza virus (AIV) in backyard and commercial ostrich flocks were first reported in the Kingdom of Saudi Arabia (KSA). The infected ostriches suffered from depression, anorexia, and diarrhea and some exhibited sudden death. A rapid AIV-group antigen detection and real-time reverse-transcription PCR (rtRT-PCR) were initially performed on cloacal and tracheal swabs collected from diseased birds. Pools from positive-tested swabs for each flock were utilized for virus isolation in specific-pathogen-free embryonating chicken eggs. H5N1 AIV was identified in the harvested allantoic fluids by hemagglutination followed by hemagglutination inhibition and rtRT-PCR. The viruses responsible for these two outbreaks were sequenced and characterized as HPAIV H5N1 (A/ostrich/Saudi Arabia/6732-3/2007 and A/ostrich/Saudi Arabia/3489-73VIR08/ 2007) from backyard and commercial flocks, respectively. Phylogenetic analysis of both isolates revealed that the two viruses belong to clade 2.2 sublineage II and cluster with the HPAIV H5N1 isolated from falcons and turkeys during 2007 in KSA.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Glicoproteínas Hemaglutininas del Virus de la Influenza
/
Struthioniformes
/
Subtipo H5N1 del Virus de la Influenza A
/
Gripe Aviar
Límite:
Animals
País/Región como asunto:
Asia
Idioma:
En
Revista:
Avian Dis
Año:
2014
Tipo del documento:
Article
Pais de publicación:
Estados Unidos