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JC virus urinary excretion and seroprevalence in natalizumab-treated multiple sclerosis patients.
Delbue, Serena; Elia, Francesca; Carloni, Camilla; Pecchenini, Valentina; Franciotta, Diego; Gastaldi, Matteo; Colombo, Elena; Signorini, Lucia; Carluccio, Silvia; Bellizzi, Anna; Bergamaschi, Roberto; Ferrante, Pasquale.
Afiliación
  • Delbue S; Department of Biomedical Surgical and Dental Sciences, University of Milano, Via Pascal, 36, Milan, 20133, Italy.
  • Elia F; Laboratory of Translational Research Fondazione Ettore Sansavini, Lugo, Italy.
  • Carloni C; Department of Biomedical Surgical and Dental Sciences, University of Milano, Via Pascal, 36, Milan, 20133, Italy.
  • Pecchenini V; Department of Biomedical Surgical and Dental Sciences, University of Milano, Via Pascal, 36, Milan, 20133, Italy.
  • Franciotta D; Department of General Neurology, National Neurological Institute C. Mondino, Pavia, Italy.
  • Gastaldi M; Department of General Neurology, National Neurological Institute C. Mondino, Pavia, Italy.
  • Colombo E; Department of General Neurology, National Neurological Institute C. Mondino, Pavia, Italy.
  • Signorini L; Department of Biomedical Surgical and Dental Sciences, University of Milano, Via Pascal, 36, Milan, 20133, Italy.
  • Carluccio S; Department of Biomedical Surgical and Dental Sciences, University of Milano, Via Pascal, 36, Milan, 20133, Italy.
  • Bellizzi A; Department of Public Health and Infectious Diseases, Sapienza University, Rome, Italy.
  • Bergamaschi R; Department of General Neurology, National Neurological Institute C. Mondino, Pavia, Italy.
  • Ferrante P; Department of Biomedical Surgical and Dental Sciences, University of Milano, Via Pascal, 36, Milan, 20133, Italy. pasquale.ferrante@unimi.it.
J Neurovirol ; 21(6): 645-52, 2015 Dec.
Article en En | MEDLINE | ID: mdl-25052191
The risk of developing progressive multifocal leukoencephalopathy (PML), as a consequence of infection/reactivation with JC virus (JCV), is consistent in natalizumab-treated multiple sclerosis (MS) patients, with 430 cases of PML reported so far. The risk of PML is higher in JCV seropositive patients, and it is recommended that only MS patients without JCV antibodies should be enrolled in the treatment postulating that they do not have JCV infection.We have studied forty-two natalizumab-treated MS patients, and urine and blood were collected monthly for up to 60 months. JCV and BK virus (BKV) DNA presence was verified using quantitative real-time PCR assays, and serum anti-JCV antibodies were measured with the Stratify and/or Stratify DxSelect tests.JCV and BKV DNA were not found in the blood samples, whereas they were found at least once in the urine of 21 of 42 (50 %) and of 25/42 (59.5 %) patients, respectively. JCV DNA urinary shedding increased up to month 24 of natalizumab treatment (45.2 %), and the effect of time was significant for JCV (p = 0.04), but not for BKV (p = 0.39). JCV viruria and seropositivity did not completely correlate, since three patients shedding JCV DNA in the urine were seronegative according to the serological tests.The results indicated that natalizumab therapy may increase the rate of JCV urinary shedding. Additionally, we confirmed that the identification of JCV carriers cannot solely rely on serological tests, but sensitive methods for viral DNA detection should be adopted to more precisely identify the truly JCV uninfected cases.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN Viral / Esclerosis Múltiple Recurrente-Remitente / Natalizumab / Factores Inmunológicos / Anticuerpos Antivirales Tipo de estudio: Prognostic_studies Límite: Adult / Female / Humans / Male / Middle aged Idioma: En Revista: J Neurovirol Asunto de la revista: NEUROLOGIA / VIROLOGIA Año: 2015 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN Viral / Esclerosis Múltiple Recurrente-Remitente / Natalizumab / Factores Inmunológicos / Anticuerpos Antivirales Tipo de estudio: Prognostic_studies Límite: Adult / Female / Humans / Male / Middle aged Idioma: En Revista: J Neurovirol Asunto de la revista: NEUROLOGIA / VIROLOGIA Año: 2015 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Estados Unidos