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Post-transcriptional regulation of autophagy in C2C12 myotubes following starvation and nutrient restoration.
Desgeorges, Marine Maud; Freyssenet, Damien; Chanon, Stéphanie; Castells, Josiane; Pugnière, Pascal; Béchet, Daniel; Peinnequin, André; Devillard, Xavier; Defour, Aurélia.
Afiliación
  • Desgeorges MM; Laboratoire de Physiologie de l'Exercice EA 4338, Université de Lyon, Université Jean Monnet, Saint Etienne, France.
  • Freyssenet D; Laboratoire de Physiologie de l'Exercice EA 4338, Université de Lyon, Université Jean Monnet, Saint Etienne, France. Electronic address: damien.freyssenet@univ-st-etienne.fr.
  • Chanon S; Laboratoire Carmen, Institut National de la Recherche Agronomique UMR1235, University Lyon-1, INSA-Lyon, F-69600 Oullins, France.
  • Castells J; Laboratoire de Physiologie de l'Exercice EA 4338, Université de Lyon, Université Jean Monnet, Saint Etienne, France.
  • Pugnière P; Pôle de Génomique, Institut de Recherche Biomédicale des Armées, Centre de Recherche du Service de Santé des Armées, La Tronche, France.
  • Béchet D; INRA UMR 1019, Unité Nutrition Humaine, St Genès Champanelle, France.
  • Peinnequin A; Pôle de Génomique, Institut de Recherche Biomédicale des Armées, Centre de Recherche du Service de Santé des Armées, La Tronche, France.
  • Devillard X; Laboratoire de Physiologie de l'Exercice EA 4338, Université de Lyon, Université Jean Monnet, Saint Etienne, France.
  • Defour A; Laboratoire de Physiologie de l'Exercice EA 4338, Université de Lyon, Université Jean Monnet, Saint Etienne, France; Center for Genetic Medicine Research, Children's National Medical Center, Washington, DC 20010, USA.
Int J Biochem Cell Biol ; 54: 208-16, 2014 Sep.
Article en En | MEDLINE | ID: mdl-25043686
In skeletal muscle, autophagy is activated in multiple physiological and pathological conditions, notably through the transcriptional regulation of autophagy-related genes by FoxO3. However, recent evidence suggests that autophagy could also be regulated by post-transcriptional mechanisms. The purpose of the study was therefore to determine the temporal regulation of transcriptional and post-transcriptional events involved in the control of autophagy during starvation (4h) and nutrient restoration (4h) in C2C12 myotubes. Starvation was associated with an activation of autophagy (decrease in mTOR activity, increase in AMPK activity and Ulk1 phosphorylation on Ser467), an increase in autophagy flux (increased LC3B-II/LC3B-I ratio, LC3B-II level and LC3B-positive punctate), and an increase in the content of autophagy-related proteins (Ulk1, Atg13, Vps34, and Atg5-Atg12 conjugate). Our data also indicated that the content of autophagy-related proteins was essentially maintained when nutrient sufficiency was restored. By contrast, mRNA level of Ulk1, Atg5, Bnip3, LC3B and Gabarapl1 did not increase in response to starvation. Accordingly, binding of FoxO3 transcription factor on LC3B promoter was only increased at the end of the starvation period, whereas mRNA levels of Atrogin1/MAFbx and MuRF1, two transcriptional targets of FoxO involved in ubiquitin-proteasome pathway, were markedly increased at this time. Together, these data provide evidence that target genes of FoxO3 are differentially regulated during starvation and that starvation of C2C12 myotubes is associated with a post-transcriptional regulation of autophagy.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Autofagia / Inanición / Procesamiento Postranscripcional del ARN / Regulación de la Expresión Génica / Músculo Esquelético / Fibras Musculares Esqueléticas / Fenómenos Fisiológicos de la Nutrición Límite: Animals Idioma: En Revista: Int J Biochem Cell Biol Asunto de la revista: BIOQUIMICA Año: 2014 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Autofagia / Inanición / Procesamiento Postranscripcional del ARN / Regulación de la Expresión Génica / Músculo Esquelético / Fibras Musculares Esqueléticas / Fenómenos Fisiológicos de la Nutrición Límite: Animals Idioma: En Revista: Int J Biochem Cell Biol Asunto de la revista: BIOQUIMICA Año: 2014 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Países Bajos