Topographical alterations in proteins I of Neisseria gonorrhoeae correlated with lipooligosaccharide variation.
Mol Microbiol
; 3(5): 637-43, 1989 May.
Article
en En
| MEDLINE
| ID: mdl-2503681
Four transformant strains of Neisseria gonorrhoeae were generated, two of which (WS3 and WS5) had protein I subclass A (P.IA) and two which (WS2 and WS4) had protein I subclass B (P.IB). Analysis of the strains demonstrated that the two P.IA-bearing strains differed in lipooligosaccharide (LOS) and H.8 antigen, as assessed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting. The WS5 strain had slow-migrating LOS and H.8 antigen, and the WS3 strain had fast-migrating LOS and H.8 antigen. The P.IB-bearing strains also had either slow-migrating LOS and H.8 antigen (WS4) or fast-migrating LOS and H.8 antigen (WS2). Structural and exposure analysis revealed that although the P.IAs were identical in the WS3 and WS5 strains, there was a slight alteration of the exposure of the proteins which correlated with altered LOS and/or H.8 antigen. The P.IBs were also shown to be structurally identical, but the LOS and/or H.8 antigen variation in these strains correlated with a more pronounced alteration in the exposure of the P.IB molecules. The differences in protein I (P.I) exposure were generally found in highly negatively charged regions of the molecule, suggesting that the immunogenicity and/or antigenicity of the P.I molecules may vary as a result of LOS and/or H.8 antigen alterations.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas de la Membrana Bacteriana Externa
/
Variación Antigénica
/
Lipopolisacáridos
/
Porinas
/
Antígenos Bacterianos
/
Neisseria gonorrhoeae
Idioma:
En
Revista:
Mol Microbiol
Asunto de la revista:
BIOLOGIA MOLECULAR
/
MICROBIOLOGIA
Año:
1989
Tipo del documento:
Article
Pais de publicación:
Reino Unido