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The quantitative architecture of centromeric chromatin.
Bodor, Dani L; Mata, João F; Sergeev, Mikhail; David, Ana Filipa; Salimian, Kevan J; Panchenko, Tanya; Cleveland, Don W; Black, Ben E; Shah, Jagesh V; Jansen, Lars Et.
Afiliación
  • Bodor DL; Instituto Gulbenkian de Ciência, Oeiras, Portugal.
  • Mata JF; Instituto Gulbenkian de Ciência, Oeiras, Portugal.
  • Sergeev M; Department of Systems Biology, Harvard Medical School, Boston, United States Renal Division, Brigham and Women's Hospital, Boston, United States.
  • David AF; Instituto Gulbenkian de Ciência, Oeiras, Portugal.
  • Salimian KJ; Department of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, United States.
  • Panchenko T; Department of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, United States.
  • Cleveland DW; Ludwig Institute for Cancer Research, University of California, San Diego, La Jolla, United States Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, United States.
  • Black BE; Department of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, United States.
  • Shah JV; Department of Systems Biology, Harvard Medical School, Boston, United States Renal Division, Brigham and Women's Hospital, Boston, United States.
  • Jansen LE; Instituto Gulbenkian de Ciência, Oeiras, Portugal ljansen@igc.gulbenkian.pt.
Elife ; 3: e02137, 2014 Jul 15.
Article en En | MEDLINE | ID: mdl-25027692
The centromere, responsible for chromosome segregation during mitosis, is epigenetically defined by CENP-A containing chromatin. The amount of centromeric CENP-A has direct implications for both the architecture and epigenetic inheritance of centromeres. Using complementary strategies, we determined that typical human centromeres contain ∼400 molecules of CENP-A, which is controlled by a mass-action mechanism. This number, despite representing only ∼4% of all centromeric nucleosomes, forms a ∼50-fold enrichment to the overall genome. In addition, although pre-assembled CENP-A is randomly segregated during cell division, this amount of CENP-A is sufficient to prevent stochastic loss of centromere function and identity. Finally, we produced a statistical map of CENP-A occupancy at a human neocentromere and identified nucleosome positions that feature CENP-A in a majority of cells. In summary, we present a quantitative view of the centromere that provides a mechanistic framework for both robust epigenetic inheritance of centromeres and the paucity of neocentromere formation.DOI: http://dx.doi.org/10.7554/eLife.02137.001.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cromatina / Centrómero Límite: Humans Idioma: En Revista: Elife Año: 2014 Tipo del documento: Article País de afiliación: Portugal Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cromatina / Centrómero Límite: Humans Idioma: En Revista: Elife Año: 2014 Tipo del documento: Article País de afiliación: Portugal Pais de publicación: Reino Unido