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Coregulated genes link sulfide:quinone oxidoreductase and arsenic metabolism in Synechocystis sp. strain PCC6803.
Nagy, Csaba I; Vass, Imre; Rákhely, Gábor; Vass, István Zoltán; Tóth, András; Duzs, Agnes; Peca, Loredana; Kruk, Jerzy; Kós, Péter B.
Afiliación
  • Nagy CI; Institute of Plant Biology, Biological Research Centre of the Hungarian Academy of Sciences, Szeged, Hungary.
  • Vass I; Institute of Plant Biology, Biological Research Centre of the Hungarian Academy of Sciences, Szeged, Hungary.
  • Rákhely G; Department of Biotechnology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary Institute of Biophysics, Biological Research Centre of the Hungarian Academy of Sciences, Szeged, Hungary.
  • Vass IZ; Institute of Plant Biology, Biological Research Centre of the Hungarian Academy of Sciences, Szeged, Hungary.
  • Tóth A; Department of Biotechnology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary Institute of Biophysics, Biological Research Centre of the Hungarian Academy of Sciences, Szeged, Hungary.
  • Duzs A; Institute of Biophysics, Biological Research Centre of the Hungarian Academy of Sciences, Szeged, Hungary.
  • Peca L; Institute of Plant Biology, Biological Research Centre of the Hungarian Academy of Sciences, Szeged, Hungary.
  • Kruk J; Department of Plant Physiology and Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland.
  • Kós PB; Institute of Plant Biology, Biological Research Centre of the Hungarian Academy of Sciences, Szeged, Hungary Department of Biotechnology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary kp@brc.hu.
J Bacteriol ; 196(19): 3430-40, 2014 Oct.
Article en En | MEDLINE | ID: mdl-25022856
Although the biogeochemistry of the two environmentally hazardous compounds arsenic and sulfide has been extensively investigated, the biological interference of these two toxic but potentially energy-rich compounds has only been hypothesized and indirectly proven. Here we provide direct evidence for the first time that in the photosynthetic model organism Synechocystis sp. strain PCC6803 the two metabolic pathways are linked by coregulated genes that are involved in arsenic transport, sulfide oxidation, and probably in sulfide-based alternative photosynthesis. Although Synechocystis sp. strain PCC6803 is an obligate photoautotrophic cyanobacterium that grows via oxygenic photosynthesis, we discovered that specific genes are activated in the presence of sulfide or arsenite to exploit the energy potentials of these chemicals. These genes form an operon that we termed suoRSCT, located on a transposable element of type IS4 on the plasmid pSYSM of the cyanobacterium. suoS (sll5036) encodes a light-dependent, type I sulfide:quinone oxidoreductase. The suoR (sll5035) gene downstream of suoS encodes a regulatory protein that belongs to the ArsR-type repressors that are normally involved in arsenic resistance. We found that this repressor has dual specificity, resulting in 200-fold induction of the operon upon either arsenite or sulfide exposure. The suoT gene encodes a transmembrane protein similar to chromate transporters but in fact functioning as an arsenite importer at permissive concentrations. We propose that the proteins encoded by the suoRSCT operon might have played an important role under anaerobic, reducing conditions on primordial Earth and that the operon was acquired by the cyanobacterium via horizontal gene transfer.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Arsénico / Quinona Reductasas / Proteínas Bacterianas / Regulación Bacteriana de la Expresión Génica / Synechocystis Idioma: En Revista: J Bacteriol Año: 2014 Tipo del documento: Article País de afiliación: Hungria Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Arsénico / Quinona Reductasas / Proteínas Bacterianas / Regulación Bacteriana de la Expresión Génica / Synechocystis Idioma: En Revista: J Bacteriol Año: 2014 Tipo del documento: Article País de afiliación: Hungria Pais de publicación: Estados Unidos