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Differentiation in MALDI-TOF MS and FTIR spectra between two pathovars of Xanthomonas oryzae.
Ge, Mengyu; Li, Bin; Wang, Li; Tao, Zhongyun; Mao, Shengfeng; Wang, Yangli; Xie, Guanlin; Sun, Guochang.
Afiliación
  • Ge M; State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310029, PR China.
  • Li B; State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310029, PR China; State Key Laboratory Cultivation Base of Subtropical Silviculture, Zhejiang Agriculture & Forestry University, Hangzhou 311300, PR China. Electronic address: libin0571@zju.edu.cn.
  • Wang L; State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310029, PR China.
  • Tao Z; State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310029, PR China.
  • Mao S; State Key Laboratory Cultivation Base of Subtropical Silviculture, Zhejiang Agriculture & Forestry University, Hangzhou 311300, PR China.
  • Wang Y; State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR China.
  • Xie G; State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310029, PR China.
  • Sun G; State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR China. Electronic address: sungc01@sina.com.
Spectrochim Acta A Mol Biomol Spectrosc ; 133: 730-4, 2014 Dec 10.
Article en En | MEDLINE | ID: mdl-24996215
Xanthomonas oryzae pv. oryzae (Xoo) and Xanthomonas oryzae pv. oryzicola (Xoc) strains are closely related phenotypically and genetically, which make it difficult to differentiate between the two pathovars based on phenotypic and DNA-based methods. In this study, a fast and accurate method was developed based on the differences in MALDI-TOF MS and FTIR spectra between the two pathovars. MALDI-TOF MS analysis revealed that 9 and 10 peaks are specific to Xoo and Xoc, respectively, which can be used as biomarkers to identify and differentiate the two closely related pathovars. Furthermore, FTIR analysis showed that there is a significant difference in both the band frequencies and absorption intensity of various functional groups between the two pathovars. In particular, the 6 peaks at 3433, 2867, 1273, 1065, 983 and 951cm(-1) were specific to the Xoo strains, while one peak at 1572cm(-1) was specific to the Xoc strains. Overall, this study gives the first attempt to identify and differentiate the two pathovars of X. oryzae based on mass and FTIR spectra, which will be helpful for the early detection and prevention of the two rice diseases caused by both X. oryzae pathovars.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oryza / Xanthomonas / Técnicas de Tipificación Bacteriana / Espectroscopía Infrarroja por Transformada de Fourier / Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción Tipo de estudio: Screening_studies Idioma: En Revista: Spectrochim Acta A Mol Biomol Spectrosc Asunto de la revista: BIOLOGIA MOLECULAR Año: 2014 Tipo del documento: Article Pais de publicación: Reino Unido
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oryza / Xanthomonas / Técnicas de Tipificación Bacteriana / Espectroscopía Infrarroja por Transformada de Fourier / Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción Tipo de estudio: Screening_studies Idioma: En Revista: Spectrochim Acta A Mol Biomol Spectrosc Asunto de la revista: BIOLOGIA MOLECULAR Año: 2014 Tipo del documento: Article Pais de publicación: Reino Unido