Measuring the rate constants of Ca²âº indicators.

Faas, Guido C; Mody, Istvan

Measuring the rate constants of Ca²âº indicators.

Faas, Guido C; Mody, Istvan.

Afiliación

Faas GC; Department of Neurology, David Geffen School of Medicine, UCLA, Los Angeles, California 90095;
Mody I; Department of Neurology, David Geffen School of Medicine, UCLA, Los Angeles, California 90095; Department of Physiology, David Geffen School of Medicine, UCLA, Los Angeles, California 90095.

Cold Spring Harb Protoc ; 2014(7): 763-7, 2014 Jul 01.

Article en En | MEDLINE | ID: mdl-24987135

RESUMEN

Fluorescent Ca(2+) indicators are widely used to measure the concentration of free Ca(2+) ([Ca(2+)](free)) in biological processes. To determine the exact kinetics of changes in [Ca(2+)](free) and the processes underlying these changes (e.g., Ca(2+) binding to Ca(2+)-binding proteins), it is necessary to know the exact binding properties of the indicator used. Here, we describe how to determine the binding rate constants (k(on) and k(off)) of Ca(2+) indicators.

Asunto(s)

Calcio/análisis; Técnicas de Química Analítica; Colorantes Fluorescentes/química; Calcio/química

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas de Química Analítica / Calcio / Colorantes Fluorescentes Idioma: En Revista: Cold Spring Harb Protoc Año: 2014 Tipo del documento: Article Pais de publicación: Estados Unidos

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