Your browser doesn't support javascript.
loading
Src as a novel therapeutic target for endometriosis.
Lawrenson, Kate; Lee, Nathan; Torres, Hugo A M; Lee, Janet M; Brueggmann, Doerthe; Rao, P Nagesh; Noushmehr, Houtan; Gayther, Simon A.
Afiliación
  • Lawrenson K; Department of Preventive Medicine, University of Southern California/Keck School of Medicine, 1450 Biggy Street, Los Angeles, CA 90033, USA. Electronic address: Kate.Lawrenson@med.usc.edu.
  • Lee N; Department of Preventive Medicine, University of Southern California/Keck School of Medicine, 1450 Biggy Street, Los Angeles, CA 90033, USA. Electronic address: nathanl@usc.edu.
  • Torres HA; Department of Genetics, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Núcleo de Apoio à Pesquisa (NAP) denominado Centro de Biologia Sistêmica Integrada (CISBi), São Paulo, Brazil.
  • Lee JM; Department of Preventive Medicine, University of Southern California/Keck School of Medicine, 1450 Biggy Street, Los Angeles, CA 90033, USA. Electronic address: janetmle@usc.edu.
  • Brueggmann D; Department of Obstetrics and Gynecology, University of Southern California/Keck School of Medicine, 1450 Biggy Street, Los Angeles, CA 90033, USA. Electronic address: brueggma@usc.edu.
  • Rao PN; Pathology and Lab Medicine, David Geffen University of California Los Angeles, 22-26 Rehab Cntr, 1000 Veteran Ave, Los Angeles, CA 90024, USA. Electronic address: nrao@ucla.edu.
  • Noushmehr H; Department of Genetics, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Núcleo de Apoio à Pesquisa (NAP) denominado Centro de Biologia Sistêmica Integrada (CISBi), São Paulo, Brazil. Electronic address: houtan@fmrp.usp.br.
  • Gayther SA; Department of Preventive Medicine, University of Southern California/Keck School of Medicine, 1450 Biggy Street, Los Angeles, CA 90033, USA. Electronic address: gayther@usc.edu.
Gynecol Oncol ; 135(1): 100-7, 2014 Oct.
Article en En | MEDLINE | ID: mdl-24972189
BACKGROUND: Endometriosis is a common condition that is associated with an increased risk of developing ovarian carcinoma. Improved in vitro models of this disease are needed to better understand how endometriosis, a benign disease, can undergo neoplastic transformation, and for the development of novel treatment strategies to prevent this progression. METHODS: We describe the generation and in vitro characterization of novel TERT immortalized ovarian endometriosis epithelial cell lines (EEC16-TERT). RESULTS: Expression of TERT alone was sufficient to immortalize endometriosis epithelial cells. TERT immortalization induces an epithelial-to-mesenchymal transition and perturbation in the expression of genes involved in the development of ovarian cancer. EEC16-TERT was non-tumorigenic when xenografted into immunocompromised mice but grew in anchorage-independent growth assays in an epidermal growth factor and hydrocortisone dependent manner. Colony formation in agar was abolished by inhibition of Src, and the Src pathway was found to be activated in human endometriosis lesions. CONCLUSIONS: This new in vitro model system mimics endometriosis and the early stages of neoplastic transformation in the development of endometriosis associated ovarian cancer. We demonstrate the potential clinical relevance of this model by identifying Src activation as a novel pathway in endometriosis that could be targeted therapeutically, perhaps as a novel strategy to manage endometriosis clinically, or to prevent the development of endometriosis-associated ovarian cancer.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades del Ovario / Neoplasias Ováricas / Transformación Celular Neoplásica / Genes src / Endometriosis Tipo de estudio: Prognostic_studies Límite: Animals / Female / Humans Idioma: En Revista: Gynecol Oncol Año: 2014 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades del Ovario / Neoplasias Ováricas / Transformación Celular Neoplásica / Genes src / Endometriosis Tipo de estudio: Prognostic_studies Límite: Animals / Female / Humans Idioma: En Revista: Gynecol Oncol Año: 2014 Tipo del documento: Article Pais de publicación: Estados Unidos