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Phototransformable fluorescent proteins: Future challenges.
Adam, Virgile; Berardozzi, Romain; Byrdin, Martin; Bourgeois, Dominique.
Afiliación
  • Adam V; Univ. Grenoble Alpes, IBS, F-38044 Grenoble, France; CNRS, IBS, F-38044 Grenoble, France; CEA, IBS, F-38044 Grenoble, France. Electronic address: virgile.adam@ibs.fr.
  • Berardozzi R; Univ. Grenoble Alpes, IBS, F-38044 Grenoble, France; CNRS, IBS, F-38044 Grenoble, France; CEA, IBS, F-38044 Grenoble, France.
  • Byrdin M; Univ. Grenoble Alpes, IBS, F-38044 Grenoble, France; CNRS, IBS, F-38044 Grenoble, France; CEA, IBS, F-38044 Grenoble, France.
  • Bourgeois D; Univ. Grenoble Alpes, IBS, F-38044 Grenoble, France; CNRS, IBS, F-38044 Grenoble, France; CEA, IBS, F-38044 Grenoble, France. Electronic address: dominique.bourgeois@ibs.fr.
Curr Opin Chem Biol ; 20: 92-102, 2014 Jun.
Article en En | MEDLINE | ID: mdl-24971562
In fluorescence microscopy, the photophysical properties of the fluorescent markers play a fundamental role. The beauty of phototransformable fluorescent proteins (PTFPs) is that some of these properties can be precisely controlled by light. A wide range of PTFPs have been developed in recent years, including photoactivatable, photoconvertible and photoswitchable fluorescent proteins. These smart labels triggered a plethora of advanced fluorescence methods to scrutinize biological cells or organisms dynamically, quantitatively and with unprecedented resolution. Despite continuous improvements, PTFPs still suffer from limitations, and mechanistic questions remain as to how these proteins precisely work.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Luminiscentes Límite: Animals / Humans Idioma: En Revista: Curr Opin Chem Biol Asunto de la revista: BIOQUIMICA Año: 2014 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Luminiscentes Límite: Animals / Humans Idioma: En Revista: Curr Opin Chem Biol Asunto de la revista: BIOQUIMICA Año: 2014 Tipo del documento: Article Pais de publicación: Reino Unido