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Chronic ethanol exposure increases the non-dominant glucocorticoid, corticosterone, in the near-term pregnant guinea pig.
Hewitt, Amy J; Dobson, Christine C; Brien, James F; Wynne-Edwards, Katherine E; Reynolds, James N.
Afiliación
  • Hewitt AJ; Department of Biomedical and Molecular Sciences, Queen's University, Kingston, ON, Canada K7L 3N6.
  • Dobson CC; Department of Biomedical and Molecular Sciences, Queen's University, Kingston, ON, Canada K7L 3N6.
  • Brien JF; Department of Biomedical and Molecular Sciences, Queen's University, Kingston, ON, Canada K7L 3N6; Centre for Neuroscience Studies, Queen's University, Kingston, ON, Canada K7L 3N6.
  • Wynne-Edwards KE; Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary Medicine, and Hotchkiss Brain Institute, University of Calgary, Calgary, AB, Canada T2N 4N1.
  • Reynolds JN; Department of Biomedical and Molecular Sciences, Queen's University, Kingston, ON, Canada K7L 3N6; Centre for Neuroscience Studies, Queen's University, Kingston, ON, Canada K7L 3N6. Electronic address: jnr@queensu.ca.
Alcohol ; 48(5): 477-81, 2014 Aug.
Article en En | MEDLINE | ID: mdl-24961542
Maternal-fetal signaling is critical for optimal fetal development and postnatal outcomes. Chronic ethanol exposure alters programming of the fetal hypothalamic-pituitary-adrenal (HPA) axis, resulting in a myriad of neurochemical and behavioral alterations in postnatal life. Based on a recent study which showed that human intra-partum fetal stress increased fetal secretion of corticosterone, the non-dominant glucocorticoid, this investigation tested the hypothesis that an established model of HPA axis programming, chronic maternal ethanol administration to the pregnant guinea pig, would result in preferential elevation of corticosterone, which is also the non-dominant glucocorticoid. Starting on gestational day (GD) 2, guinea pigs received oral administration of ethanol (4 g/kg maternal body weight/day) or isocaloric-sucrose/pair-feeding. Each treatment was administered daily and continued until GD 45, 55, or 65 (approximately 3 days pre-term), when pregnant animals were euthanized and fetuses delivered by Caesarean section. Maternal and fetal plasma samples were collected. After sample preparation (protein precipitation and C-18 solid phase extraction), plasma cortisol and corticosterone concentrations were determined simultaneously by liquid chromatography coupled to tandem mass spectrometry. As predicted, chronic ethanol exposure increased both fetal and maternal plasma corticosterone concentration in late gestation. In contrast, plasma cortisol did not differ across maternal treatments in maternal or fetal samples. The plasma concentration of both maternal glucocorticoids increased with gestational age. Thus, corticosterone, the non-dominant glucocorticoid, but not cortisol, was elevated by chronic ethanol exposure, which may have effects on HPA function in later life.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Preñez / Corticosterona / Etanol Tipo de estudio: Prognostic_studies Límite: Animals / Pregnancy Idioma: En Revista: Alcohol Asunto de la revista: TRANSTORNOS RELACIONADOS COM SUBSTANCIAS Año: 2014 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Preñez / Corticosterona / Etanol Tipo de estudio: Prognostic_studies Límite: Animals / Pregnancy Idioma: En Revista: Alcohol Asunto de la revista: TRANSTORNOS RELACIONADOS COM SUBSTANCIAS Año: 2014 Tipo del documento: Article Pais de publicación: Estados Unidos