A versatile biosensing system for DNA-related enzyme activity assay via the synthesis of silver nanoclusters using enzymatically-generated DNA as template.

Yuan, Yijia; Li, Wenhua; Liu, Zhuoliang; Nie, Zhou; Huang, Yan; Yao, Shouzhuo

Yuan, Yijia; Li, Wenhua; Liu, Zhuoliang; Nie, Zhou; Huang, Yan; Yao, Shouzhuo.

Afiliación

Yuan Y; State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China.
Li W; State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China.
Liu Z; State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China.
Nie Z; State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China. Electronic address: niezhou.hnu@gmail.com.
Huang Y; State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China.
Yao S; State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China.

Biosens Bioelectron ; 61: 321-7, 2014 Nov 15.

Article en En | MEDLINE | ID: mdl-24907540

Asunto(s)

Técnicas Biosensibles/métodos; ADN/química; Desoxirribonucleasa EcoRI/análisis; Pruebas de Enzimas/métodos; Exodesoxirribonucleasas/análisis; Nanoestructuras/química; Plata/química; ADN/metabolismo; Desoxirribonucleasa EcoRI/metabolismo; Exodesoxirribonucleasas/metabolismo; Límite de Detección; Nanoestructuras/ultraestructura; Polimerizacion; Espectrometría de Fluorescencia/métodos

Palabras clave

DNA-templated synthesis; Label-free detection; Nuclease activity assay; Silver nanocluster; Terminal deoxynucleotidyl transferase

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plata / ADN / Técnicas Biosensibles / Desoxirribonucleasa EcoRI / Nanoestructuras / Exodesoxirribonucleasas / Pruebas de Enzimas Tipo de estudio: Evaluation_studies Idioma: En Revista: Biosens Bioelectron Asunto de la revista: BIOTECNOLOGIA Año: 2014 Tipo del documento: Article Pais de publicación: Reino Unido

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