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Quantitative temporal viromics: an approach to investigate host-pathogen interaction.
Weekes, Michael P; Tomasec, Peter; Huttlin, Edward L; Fielding, Ceri A; Nusinow, David; Stanton, Richard J; Wang, Eddie C Y; Aicheler, Rebecca; Murrell, Isa; Wilkinson, Gavin W G; Lehner, Paul J; Gygi, Steven P.
Afiliación
  • Weekes MP; Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA; Cambridge Institute for Medical Research, University of Cambridge, Hills Road, Cambridge CB2 0XY, UK. Electronic address: mpw1001@cam.ac.uk.
  • Tomasec P; School of Medicine, Cardiff University, Tenovus Building, Heath Park, Cardiff CF14 4XX, UK.
  • Huttlin EL; Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA.
  • Fielding CA; School of Medicine, Cardiff University, Tenovus Building, Heath Park, Cardiff CF14 4XX, UK.
  • Nusinow D; Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA.
  • Stanton RJ; School of Medicine, Cardiff University, Tenovus Building, Heath Park, Cardiff CF14 4XX, UK.
  • Wang ECY; School of Medicine, Cardiff University, Tenovus Building, Heath Park, Cardiff CF14 4XX, UK.
  • Aicheler R; School of Medicine, Cardiff University, Tenovus Building, Heath Park, Cardiff CF14 4XX, UK.
  • Murrell I; School of Medicine, Cardiff University, Tenovus Building, Heath Park, Cardiff CF14 4XX, UK.
  • Wilkinson GWG; School of Medicine, Cardiff University, Tenovus Building, Heath Park, Cardiff CF14 4XX, UK.
  • Lehner PJ; Cambridge Institute for Medical Research, University of Cambridge, Hills Road, Cambridge CB2 0XY, UK.
  • Gygi SP; Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA. Electronic address: steven_gygi@hms.harvard.edu.
Cell ; 157(6): 1460-1472, 2014 Jun 05.
Article en En | MEDLINE | ID: mdl-24906157
A systematic quantitative analysis of temporal changes in host and viral proteins throughout the course of a productive infection could provide dynamic insights into virus-host interaction. We developed a proteomic technique called "quantitative temporal viromics" (QTV), which employs multiplexed tandem-mass-tag-based mass spectrometry. Human cytomegalovirus (HCMV) is not only an important pathogen but a paradigm of viral immune evasion. QTV detailed how HCMV orchestrates the expression of >8,000 cellular proteins, including 1,200 cell-surface proteins to manipulate signaling pathways and counterintrinsic, innate, and adaptive immune defenses. QTV predicted natural killer and T cell ligands, as well as 29 viral proteins present at the cell surface, potential therapeutic targets. Temporal profiles of >80% of HCMV canonical genes and 14 noncanonical HCMV open reading frames were defined. QTV is a powerful method that can yield important insights into viral infection and is applicable to any virus with a robust in vitro model.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Virología / Infecciones por Citomegalovirus / Citomegalovirus / Proteómica / Interacciones Huésped-Patógeno Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Cell Año: 2014 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Virología / Infecciones por Citomegalovirus / Citomegalovirus / Proteómica / Interacciones Huésped-Patógeno Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Cell Año: 2014 Tipo del documento: Article Pais de publicación: Estados Unidos