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Second worldwide proficiency study on variable number of tandem repeats typing of Mycobacterium tuberculosis complex.
de Beer, J L; Ködmön, C; van Ingen, J; Supply, P; van Soolingen, D.
Afiliación
  • de Beer JL; National Tuberculosis Reference Laboratory, Center for Infectious Diseases Research, Diagnostics and Screening, Centre for Infectious Disease Control, National Institute for Public Health and the Environment, Bilthoven, The Netherlands.
  • Ködmön C; European Centre for Disease Prevention and Control, Stockholm, Sweden.
  • van Ingen J; Department of Medical Microbiology, Radboud University Medical Centre, Nijmegen, The Netherlands.
  • Supply P; Institut National de la Santé et de la Recherche Médicale, Lille, France.
  • van Soolingen D; National Tuberculosis Reference Laboratory, Center for Infectious Diseases Research, Diagnostics and Screening, Centre for Infectious Disease Control, National Institute for Public Health and the Environment, Bilthoven, The Netherlands.
Int J Tuberc Lung Dis ; 18(5): 594-600, 2014 May.
Article en En | MEDLINE | ID: mdl-24903798
BACKGROUND: The quality of variable number of tandem repeats (VNTR) typing of Mycobacterium tuberculosis was first investigated in 2009 in 37 laboratories worldwide. The results revealed an inter- and intra-laboratory reproducibility of respectively 60% and 72%. These data spurred an improvement in laboratory-specific assays and global standardisation of VNTR typing. OBJECTIVE: To measure the effects of the technical improvements and increased standardisation, a test panel consisting of 30 M. tuberculosis complex DNA samples was distributed for VNTR typing in 41 participating laboratories from 36 countries. RESULTS: The inter- and intra-laboratory reproducibility increased overall to respectively 78% and 88%. The 33 laboratories that participated in both the first and second proficiency studies improved their inter- and intra-laboratory reproducibility from 62% and 72% to respectively 79% and 88%. The largest improvement in reproducibility was detected in 10 laboratories that use an in-house polymerase chain reaction technique and perform amplicon sizing using gel electrophoresis. Detailed error analysis revealed a reduction in the number of systematic errors, sample exchange events and non-amplifiable loci. CONCLUSION: This second worldwide proficiency study indicates a substantial increase in the reproducibility of VNTR typing of M. tuberculosis. This will contribute to a more meaningful interpretation of molecular epidemiological and phylogenetic studies on the M. tuberculosis complex.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN Bacteriano / Técnicas de Tipificación Bacteriana / Repeticiones de Minisatélite / Ensayos de Aptitud de Laboratorios / Mycobacterium tuberculosis Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Int J Tuberc Lung Dis Año: 2014 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Francia
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN Bacteriano / Técnicas de Tipificación Bacteriana / Repeticiones de Minisatélite / Ensayos de Aptitud de Laboratorios / Mycobacterium tuberculosis Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Int J Tuberc Lung Dis Año: 2014 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Francia