Biofunctionalized gold nanoparticle-conducting polymer nanocomposite based bioelectrode for CRP detection.
Appl Biochem Biotechnol
; 174(3): 984-97, 2014 Oct.
Article
en En
| MEDLINE
| ID: mdl-24894662
An electrochemical impedance immunosensing method for the detection and quantification of C-reactive protein (αCRP) in phosphate buffered saline (PBS) is demonstrated. The protein antibody, Ab-αCRP, has been covalently immobilized on a platform comprising of electrochemically deposited 3-mercaptopropionic acid-capped gold nanoparticles Au(MPA)-polypyrrole (PPy) nanocomposite film of controlled thickness onto an indium tin oxide-coated glass plate. The free carboxyl groups present on the nanocomposite film have been used to site-specifically immobilize the Ab-αCRP biomolecules through a stable acyl amino ester intermediate generated by N-(3-dimethylaminopropyl)-N'-ethyl carbodiimide hydrochloride and N-hydroxysuccinimide. The nanocomposite film was characterized by atomic force microscopy, high-resolution transmission electron microscopy, Fourier transform infrared spectroscopy, and electrochemical techniques. The bioelectrode was electrochemically analyzed using modified Randles circuit in terms of constant phase element (CPE), electron transfer resistance (R et), and Warburg impedance (Z w). The value of n, a CPE exponent used as a gauge of heterogeneity, for the Au-PPy nanocomposite film was found to be 0.56 which is indicative of a rather rough morphology and porous structure. A linear relationship between the increased ∆R et values and the logarithmic value of protein antigen, Ag-αCRP, concentrations was found in the range of 10 ng to 10 µg mL(-1) with a R et sensitivity of 46.27 Ω cm(2)/decade of [Ag-αCRP] in PBS (pH 7.4).
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteína C-Reactiva
/
Técnicas Biosensibles
/
Nanopartículas del Metal
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
Appl Biochem Biotechnol
Año:
2014
Tipo del documento:
Article
País de afiliación:
India
Pais de publicación:
Estados Unidos