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Oxidation of cellular amino acid pools leads to cytotoxic mistranslation of the genetic code.
Bullwinkle, Tammy J; Reynolds, Noah M; Raina, Medha; Moghal, Adil; Matsa, Eleftheria; Rajkovic, Andrei; Kayadibi, Huseyin; Fazlollahi, Farbod; Ryan, Christopher; Howitz, Nathaniel; Faull, Kym F; Lazazzera, Beth A; Ibba, Michael.
Afiliación
  • Bullwinkle TJ; Department of Microbiology, Ohio State University, Columbus, United States.
  • Reynolds NM; Department of Microbiology, Ohio State University, Columbus, United States.
  • Raina M; Center for RNA Biology, Ohio State University, Columbus, United States.
  • Moghal A; Center for RNA Biology, Ohio State University, Columbus, United States.
  • Matsa E; Department of Microbiology, Ohio State University, Columbus, United States.
  • Rajkovic A; Center for RNA Biology, Ohio State University, Columbus, United States.
  • Kayadibi H; Pasarow Mass Spectrometry Laboratory, Semel Institute for Neuroscience and Human Behavior, University of California, Los Angeles, United States.
  • Fazlollahi F; Pasarow Mass Spectrometry Laboratory, Semel Institute for Neuroscience and Human Behavior, University of California, Los Angeles, United States.
  • Ryan C; Pasarow Mass Spectrometry Laboratory, Semel Institute for Neuroscience and Human Behavior, University of California, Los Angeles, United States.
  • Howitz N; Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, Los Angeles, United States.
  • Faull KF; Pasarow Mass Spectrometry Laboratory, Semel Institute for Neuroscience and Human Behavior, University of California, Los Angeles, United States.
  • Lazazzera BA; Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, Los Angeles, United States.
  • Ibba M; Department of Microbiology, Ohio State University, Columbus, United States.
Elife ; 32014 Jun 02.
Article en En | MEDLINE | ID: mdl-24891238
Aminoacyl-tRNA synthetases use a variety of mechanisms to ensure fidelity of the genetic code and ultimately select the correct amino acids to be used in protein synthesis. The physiological necessity of these quality control mechanisms in different environments remains unclear, as the cost vs benefit of accurate protein synthesis is difficult to predict. We show that in Escherichia coli, a non-coded amino acid produced through oxidative damage is a significant threat to the accuracy of protein synthesis and must be cleared by phenylalanine-tRNA synthetase in order to prevent cellular toxicity caused by mis-synthesized proteins. These findings demonstrate how stress can lead to the accumulation of non-canonical amino acids that must be excluded from the proteome in order to maintain cellular viability.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oxígeno / Código Genético / Aminoácidos / Aminoacil-ARNt Sintetasas Tipo de estudio: Prognostic_studies Idioma: En Revista: Elife Año: 2014 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oxígeno / Código Genético / Aminoácidos / Aminoacil-ARNt Sintetasas Tipo de estudio: Prognostic_studies Idioma: En Revista: Elife Año: 2014 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido