Your browser doesn't support javascript.
loading
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm, Benjamin G; Mandad, Sunit; Truckenbrodt, Sven; Kröhnert, Katharina; Schäfer, Christina; Rammner, Burkhard; Koo, Seong Joo; Claßen, Gala A; Krauss, Michael; Haucke, Volker; Urlaub, Henning; Rizzoli, Silvio O.
Afiliación
  • Wilhelm BG; Department of Neuro- and Sensory Physiology, University of Göttingen Medical Center, European Neuroscience Institute, Cluster of Excellence Nanoscale Microscopy and Molecular Physiology of the Brain, Göttingen, Germany. International Max Planck Research School Neurosciences, 37077 Göttingen, Germany
  • Mandad S; Bioanalytical Mass Spectrometry Group, Max-Planck-Institute for Biophysical Chemistry, 37077 Göttingen, Germany.
  • Truckenbrodt S; Department of Neuro- and Sensory Physiology, University of Göttingen Medical Center, European Neuroscience Institute, Cluster of Excellence Nanoscale Microscopy and Molecular Physiology of the Brain, Göttingen, Germany. International Max Planck Research School Molecular Biology, 37077 Göttingen, Ger
  • Kröhnert K; Department of Neuro- and Sensory Physiology, University of Göttingen Medical Center, European Neuroscience Institute, Cluster of Excellence Nanoscale Microscopy and Molecular Physiology of the Brain, Göttingen, Germany.
  • Schäfer C; Department of Neuro- and Sensory Physiology, University of Göttingen Medical Center, European Neuroscience Institute, Cluster of Excellence Nanoscale Microscopy and Molecular Physiology of the Brain, Göttingen, Germany.
  • Rammner B; Department of Neuro- and Sensory Physiology, University of Göttingen Medical Center, European Neuroscience Institute, Cluster of Excellence Nanoscale Microscopy and Molecular Physiology of the Brain, Göttingen, Germany.
  • Koo SJ; Leibniz Institut für Molekulare Pharmakologie, Department of Molecular Pharmacology and Cell Biology, Robert-Rössle-Strasse 10, 13125 Berlin, Germany.
  • Claßen GA; Leibniz Institut für Molekulare Pharmakologie, Department of Molecular Pharmacology and Cell Biology, Robert-Rössle-Strasse 10, 13125 Berlin, Germany.
  • Krauss M; Leibniz Institut für Molekulare Pharmakologie, Department of Molecular Pharmacology and Cell Biology, Robert-Rössle-Strasse 10, 13125 Berlin, Germany.
  • Haucke V; Leibniz Institut für Molekulare Pharmakologie, Department of Molecular Pharmacology and Cell Biology, Robert-Rössle-Strasse 10, 13125 Berlin, Germany.
  • Urlaub H; Bioanalytical Mass Spectrometry Group, Max-Planck-Institute for Biophysical Chemistry, 37077 Göttingen, Germany. Bioanalytics, Department of Clinical Chemistry, University Medical Center Göttingen, 37075 Göttingen, Germany.
  • Rizzoli SO; Department of Neuro- and Sensory Physiology, University of Göttingen Medical Center, European Neuroscience Institute, Cluster of Excellence Nanoscale Microscopy and Molecular Physiology of the Brain, Göttingen, Germany. srizzol@gwdg.de.
Science ; 344(6187): 1023-8, 2014 May 30.
Article en En | MEDLINE | ID: mdl-24876496
Synaptic vesicle recycling has long served as a model for the general mechanisms of cellular trafficking. We used an integrative approach, combining quantitative immunoblotting and mass spectrometry to determine protein numbers; electron microscopy to measure organelle numbers, sizes, and positions; and super-resolution fluorescence microscopy to localize the proteins. Using these data, we generated a three-dimensional model of an "average" synapse, displaying 300,000 proteins in atomic detail. The copy numbers of proteins involved in the same step of synaptic vesicle recycling correlated closely. In contrast, copy numbers varied over more than three orders of magnitude between steps, from about 150 copies for the endosomal fusion proteins to more than 20,000 for the exocytotic ones.
Asunto(s)
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vesículas Sinápticas / Sinaptosomas / Encéfalo / Terminales Presinápticos / Proteínas de Transporte Vesicular Límite: Animals Idioma: En Revista: Science Año: 2014 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vesículas Sinápticas / Sinaptosomas / Encéfalo / Terminales Presinápticos / Proteínas de Transporte Vesicular Límite: Animals Idioma: En Revista: Science Año: 2014 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Estados Unidos