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Localization of protein kinase C isoforms in the optic pathway of mouse embryos and their role in axon routing at the optic chiasm.
Wang, Liqing; Lam, Joyce Shi-Ying; Zhao, Hui; Wang, Jun; Chan, Sun-On.
Afiliación
  • Wang L; School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China; Department of Neurology, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong, China. Electronic address: merrysums@gmail.com.
  • Lam JS; School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China. Electronic address: joyce.lam@cuhk.edu.hk.
  • Zhao H; School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China. Electronic address: zhaohui@cuhk.edu.hk.
  • Wang J; Department of Anatomy and Embryology, School of Basic Medical Sciences, Peking University, Beijing, China. Electronic address: wangjun74008@bjmu.edu.cn.
  • Chan SO; School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China. Electronic address: sunonchan@cuhk.edu.hk.
Brain Res ; 1575: 22-32, 2014 Aug 05.
Article en En | MEDLINE | ID: mdl-24863469
Protein kinase C (PKC) plays a key role in many receptor-mediated signaling pathways that regulate cell growth and development. However, its roles in guiding axon growth and guidance in developing neural pathways are largely unknown. To investigate possible functions of PKC in the growth and guidance of axons in the optic chiasm, we first determined the localization of major PKC isoforms in the retinofugal pathway of mouse embryos, at the stage when axons navigate through the midline. Results showed that PKC was expressed in isoform specific patterns in the pathway. PKC-α immunoreactivity was detected in the chiasm and the optic tract. PKC-ßΙΙ was strong in the optic stalk but was attenuated on axons in the diencephalon. Immunostaining for PKC-ε showed a colocalization in the chiasmatic neurons that express a surface antigen stage specific embryonic antigen-1 (SSEA-1). These chiasmatic neurons straddled the midline of the optic chiasm, and have been shown in earlier studies a role in regulation of axon growth and guidance. Expression levels of PKC-ßΙ, -δ and -γ were barely detectable in the pathway. Blocking of PKC signaling with Ro-32-0432, an inhibitor specific for PKC-α and -ß at nanomolar concentration, produced a dramatic reduction of ipsilateral axons from both nasal retina and temporal crescent. We conclude from these studies that PKC-α and -ßΙΙ are the predominant forms in the developing optic pathway, whereas PKC-ε is the major form in the chiasmatic neurons. Furthermore, PKC-α and -ßΙΙ are likely involved in signaling pathways triggered by inhibitory molecules at the midline that guide optic axons to the uncrossed pathway.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Quiasma Óptico / Células Ganglionares de la Retina / Axones / Vías Visuales / Proteína Quinasa C Límite: Animals Idioma: En Revista: Brain Res Año: 2014 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Quiasma Óptico / Células Ganglionares de la Retina / Axones / Vías Visuales / Proteína Quinasa C Límite: Animals Idioma: En Revista: Brain Res Año: 2014 Tipo del documento: Article Pais de publicación: Países Bajos