Roles of chromatin remodelers in maintenance mechanisms of multipotency of mouse trunk neural crest cells in the formation of neural crest-derived stem cells.

Fujita, Kyohei; Ogawa, Ryuhei; Kawawaki, Syunsaku; Ito, Kazuo

Fujita, Kyohei; Ogawa, Ryuhei; Kawawaki, Syunsaku; Ito, Kazuo.

Afiliación

Fujita K; Department of Biological Sciences, Graduate School of Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka 560-0043, Japan.
Ogawa R; Department of Biological Sciences, Graduate School of Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka 560-0043, Japan.
Kawawaki S; Department of Biological Sciences, Graduate School of Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka 560-0043, Japan.
Ito K; Department of Biological Sciences, Graduate School of Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka 560-0043, Japan. Electronic address: itokazuo@bio.sci.osaka-u.ac.jp.

Mech Dev ; 133: 126-45, 2014 Aug.

Article en En | MEDLINE | ID: mdl-24836203

RESUMEN

We analyzed roles of two chromatin remodelers, Chromodomain Helicase DNA-binding protein 7 (CHD7) and SWItch/Sucrose NonFermentable-B (SWI/SNF-B), and Bone Morphogenetic Protein (BMP)/Wnt signaling in the maintenance of the multipotency of mouse trunk neural crest cells, leading to the formation of mouse neural crest-derived stem cells (mouse NCSCs). CHD7 was expressed in the undifferentiated neural crest cells and in the dorsal root ganglia (DRG) and sciatic nerve, typical tissues containing NCSCs. BMP/Wnt signaling stimulated the expression of CHD7 and participated in maintaining the multipotency of neural crest cells. Furthermore, the promotion of CHD7 expression maintained the multipotency of these cells. The inhibition of CHD7 and SWI/SNF-B expression significantly suppressed the maintenance of the multipotency of these cells. In addition, BMP/Wnt treatment promoted CHD7 expression and caused the increase of the percentage of multipotent cells in DRG. Thus, the present data suggest that the chromatin remodelers as well as BMP/Wnt signaling play essential roles in the maintenance of the multipotency of mouse trunk neural crest cells and in the formation of mouse NCSCs.

Asunto(s)

Ensamble y Desensamble de Cromatina; Cresta Neural/citología; Células-Madre Neurales/citología; Animales; Apoptosis; Proteínas Morfogenéticas Óseas/metabolismo; Diferenciación Celular; Proliferación Celular; Células Cultivadas; Proteínas Cromosómicas no Histona/genética; Proteínas Cromosómicas no Histona/metabolismo; Proteínas de Unión al ADN/antagonistas & inhibidores; Proteínas de Unión al ADN/genética; Proteínas de Unión al ADN/metabolismo; Células Madre Embrionarias/citología; Células Madre Embrionarias/metabolismo; Ganglios Espinales/citología; Ganglios Espinales/embriología; Ganglios Espinales/metabolismo; Regulación del Desarrollo de la Expresión Génica; Ratones; Células Madre Multipotentes/citología; Células Madre Multipotentes/metabolismo; Cresta Neural/metabolismo; Células-Madre Neurales/metabolismo; ARN Interferente Pequeño/genética; Receptores de Factor de Crecimiento Nervioso/genética; Receptores de Factor de Crecimiento Nervioso/metabolismo; Factores de Transcripción SOXE/genética; Factores de Transcripción SOXE/metabolismo; Nervio Ciático/citología; Nervio Ciático/embriología; Nervio Ciático/metabolismo; Transducción de Señal; Factores de Transcripción/genética; Factores de Transcripción/metabolismo; Vía de Señalización Wnt

Palabras clave

BMP/Wnt signaling; Chromatin remodeler; Mouse; Neural crest-derived stem cells

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ensamble y Desensamble de Cromatina / Células-Madre Neurales / Cresta Neural Tipo de estudio: Prognostic_studies Idioma: En Revista: Mech Dev Asunto de la revista: EMBRIOLOGIA Año: 2014 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Irlanda

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