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Therapeutic angiogenesis in a murine model of limb ischemia by recombinant periostin and its fasciclin I domain.
Kim, Ba Reun; Jang, Il Ho; Shin, Sang Hun; Kwon, Yang Woo; Heo, Soon Chul; Choi, Eun-Jung; Lee, Jung Sub; Kim, Jae Ho.
Afiliación
  • Kim BR; Medical Research Center for Ischemic Tissue Regeneration, School of Medicine, Pusan National University, Yangsan 626-870, Gyeongsangnam-do, Republic of Korea; Department of Physiology, School of Medicine, Pusan National University, Yangsan 626-870, Gyeongsangnam-do, Republic of Korea.
  • Jang IH; Department of Physiology, School of Medicine, Pusan National University, Yangsan 626-870, Gyeongsangnam-do, Republic of Korea.
  • Shin SH; Medical Research Center for Ischemic Tissue Regeneration, School of Medicine, Pusan National University, Yangsan 626-870, Gyeongsangnam-do, Republic of Korea; Department of Physiology, School of Medicine, Pusan National University, Yangsan 626-870, Gyeongsangnam-do, Republic of Korea.
  • Kwon YW; Medical Research Center for Ischemic Tissue Regeneration, School of Medicine, Pusan National University, Yangsan 626-870, Gyeongsangnam-do, Republic of Korea; Department of Physiology, School of Medicine, Pusan National University, Yangsan 626-870, Gyeongsangnam-do, Republic of Korea.
  • Heo SC; Medical Research Center for Ischemic Tissue Regeneration, School of Medicine, Pusan National University, Yangsan 626-870, Gyeongsangnam-do, Republic of Korea; Department of Physiology, School of Medicine, Pusan National University, Yangsan 626-870, Gyeongsangnam-do, Republic of Korea.
  • Choi EJ; Medical Research Center for Ischemic Tissue Regeneration, School of Medicine, Pusan National University, Yangsan 626-870, Gyeongsangnam-do, Republic of Korea; Department of Physiology, School of Medicine, Pusan National University, Yangsan 626-870, Gyeongsangnam-do, Republic of Korea.
  • Lee JS; Department of Orthopaedic Surgery, School of Medicine, Pusan National University, Yangsan 626-870, Gyeongsangnam-do, Republic of Korea.
  • Kim JH; Medical Research Center for Ischemic Tissue Regeneration, School of Medicine, Pusan National University, Yangsan 626-870, Gyeongsangnam-do, Republic of Korea; Department of Physiology, School of Medicine, Pusan National University, Yangsan 626-870, Gyeongsangnam-do, Republic of Korea; Research Insti
Biochim Biophys Acta ; 1842(9): 1324-32, 2014 Sep.
Article en En | MEDLINE | ID: mdl-24834847
Periostin, an extracellular matrix protein, is expressed in injured tissues, such as the heart with myocardial infarction, and promotes angiogenesis and tissue repair. However, the molecular mechanism associated with periostin-stimulated angiogenesis and tissue repair is still unclear. In order to clarify the role of periostin in neovascularization, we examined the effect of periostin in angiogenic potentials of human endothelial colony forming cells (ECFCs) in vitro and in an ischemic limb animal model. Recombinant periostin protein stimulated the migration and tube formation of ECFCs. To identify the functional domains of periostin implicated in angiogenesis, five fragments of periostin, including four repeating FAS-1 domains and a carboxyl terminal domain, were expressed in Escherichia coli and purified to homogeneity. Of the five different domains, the first FAS-1 domain stimulated the migration and tube formation of human ECFCs as potent as the whole periostin. Chemotactic migration of ECFCs induced by the full length and the first FAS-1 domain of periostin was abrogated by blocking antibodies against ß3 and ß5 integrins. Intramuscular injection of the full length and the first FAS-1 domain of periostin into the ischemic hindlimb of mice attenuated severe limb loss and promoted blood perfusion and homing of intravenously administered ECFCs to the ischemic limb. These results suggest that the first FAS-1 domain is responsible for periostin-induced migration and angiogenesis and it can be used as a therapeutic tool for treatment of peripheral artery occlusive disease by stimulating homing of ECFCs.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Recombinantes / Endotelio Vascular / Moléculas de Adhesión Celular Neuronal / Moléculas de Adhesión Celular / Modelos Animales de Enfermedad / Isquemia / Neovascularización Patológica Tipo de estudio: Prognostic_studies Límite: Animals / Humans / Male Idioma: En Revista: Biochim Biophys Acta Año: 2014 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
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Imprimir
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Recombinantes / Endotelio Vascular / Moléculas de Adhesión Celular Neuronal / Moléculas de Adhesión Celular / Modelos Animales de Enfermedad / Isquemia / Neovascularización Patológica Tipo de estudio: Prognostic_studies Límite: Animals / Humans / Male Idioma: En Revista: Biochim Biophys Acta Año: 2014 Tipo del documento: Article Pais de publicación: Países Bajos