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[Antigenic determinants of the constant region of human immunoglobin kappa-chain detected by monoclonal antibodies]. / Antigennye determinanty postoiannogo domena kappa-tsepei immunoglobulinov cheloveka, vyiavliaemye s pomoshch'iu monoklonal'nykh antitel.
Mol Biol (Mosk) ; 23(5): 1227-33, 1989.
Article en Ru | MEDLINE | ID: mdl-2481811
Ten different monoclonal antibody (monAB) preparations reacting with human IgL chains of the kappa type have been obtained. Nine of the monAB interacted with the kappa-chain C domain, whereas only one monAB reacted with the V domain. It has been determined that monAB against the C domain react with three different epitopes. One epitope is expressed on intact Ig molecules as well as on isolated kappa-chains, whereas the other two epitopes are found only on isolated kappa-chains. The expression of these epitopes in 40 different myeloma kappa-chain preparations belonging to four various subgroups was studied. The level of this C domain epitope expression has been shown to depend on the variable subgroups of kappa-chains indicating a close association between V and C domains. This association leads to the alteration of antigenic activity of some C domain epitopes. The alterations are thought to be local because, as a rule, they involve only one of the three epitopes.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Regiones Constantes de Inmunoglobulina / Cadenas kappa de Inmunoglobulina / Anticuerpos Monoclonales / Epítopos Límite: Humans Idioma: Ru Revista: Mol Biol (Mosk) Año: 1989 Tipo del documento: Article Pais de publicación: Rusia
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Regiones Constantes de Inmunoglobulina / Cadenas kappa de Inmunoglobulina / Anticuerpos Monoclonales / Epítopos Límite: Humans Idioma: Ru Revista: Mol Biol (Mosk) Año: 1989 Tipo del documento: Article Pais de publicación: Rusia