CD20 positive human B lymphocytes separated with the magnetic cell sorter (MACS) can be induced to proliferation and antibody secretion in vitro.
J Immunol Methods
; 125(1-2): 19-28, 1989 Dec 20.
Article
en En
| MEDLINE
| ID: mdl-2481694
A method is described for the efficient purification of human B lymphocytes from peripheral blood by magnetic separation. Biotinylated, superparamagnetic particles were coupled to target cells by fluorescein isothiocyanate conjugated avidin and biotinylated monoclonal antibodies directed against cell surface antigens. This combination permitted flow cytometric control of the magnetic separation. Ficoll-Paque-separated peripheral blood mononuclear cells were first eliminated from monocytes by leucine-methyl ester treatment. B cells were enriched to 97% after magnetic depletion of CD3-positive T cells and magnetic enrichment of CD20-positive B cells. The separated B cells could be induced to proliferation and antibody production by various in vitro stimuli.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Linfocitos B
/
Antígenos de Diferenciación de Linfocitos B
/
Separación Celular
/
Células Productoras de Anticuerpos
Límite:
Humans
Idioma:
En
Revista:
J Immunol Methods
Año:
1989
Tipo del documento:
Article
Pais de publicación:
Países Bajos