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Orientation of llama antibodies strongly increases sensitivity of biosensors.
Trilling, Anke K; Hesselink, Thamara; van Houwelingen, Adèle; Cordewener, Jan H G; Jongsma, Maarten A; Schoffelen, Sanne; van Hest, Jan C M; Zuilhof, Han; Beekwilder, Jules.
Afiliación
  • Trilling AK; Plant Research International, Wageningen, The Netherlands; Laboratory of Organic Chemistry, Wageningen University, Wageningen, The Netherlands.
  • Hesselink T; Plant Research International, Wageningen, The Netherlands.
  • van Houwelingen A; Plant Research International, Wageningen, The Netherlands.
  • Cordewener JH; Plant Research International, Wageningen, The Netherlands.
  • Jongsma MA; Plant Research International, Wageningen, The Netherlands.
  • Schoffelen S; Department of Bioorganic Chemistry, Institute for Molecules and Materials, Radboud University Nijmegen, Nijmegen, The Netherlands.
  • van Hest JC; Department of Bioorganic Chemistry, Institute for Molecules and Materials, Radboud University Nijmegen, Nijmegen, The Netherlands.
  • Zuilhof H; Laboratory of Organic Chemistry, Wageningen University, Wageningen, The Netherlands; Department of Chemical and Materials Engineering, King Abdulaziz University, Jeddah, Saudi Arabia.
  • Beekwilder J; Plant Research International, Wageningen, The Netherlands. Electronic address: jules.beekwilder@wur.nl.
Biosens Bioelectron ; 60: 130-6, 2014 Oct 15.
Article en En | MEDLINE | ID: mdl-24793095
Sensitivity of biosensors depends on the orientation of bio-receptors on the sensor surface. The objective of this study was to organize bio-receptors on surfaces in a way that their analyte binding site is exposed to the analyte solution. VHH proteins recognizing foot-and-mouth disease virus (FMDV) were used for making biosensors, and azides were introduced in the VHH to function as bioorthogonal reactive groups. The importance of the orientation of bio-receptors was addressed by comparing sensors with randomly oriented VHH (with multiple exposed azide groups) to sensors with uniformly oriented VHH (with only a single azide group). A surface plasmon resonance (SPR) chip exposing cyclooctyne was reacted to azide functionalized VHH domains, using click chemistry. Comparison between randomly and uniformly oriented bio-receptors showed up to 800-fold increase in biosensor sensitivity. This technique may increase the containment of infectious diseases such as FMDV as its strongly enhanced sensitivity may facilitate early diagnostics.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Camélidos del Nuevo Mundo / Inmunoensayo / Técnicas Biosensibles / Resonancia por Plasmón de Superficie / Virus de la Fiebre Aftosa / Anticuerpos Antivirales Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: Biosens Bioelectron Asunto de la revista: BIOTECNOLOGIA Año: 2014 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Reino Unido
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Camélidos del Nuevo Mundo / Inmunoensayo / Técnicas Biosensibles / Resonancia por Plasmón de Superficie / Virus de la Fiebre Aftosa / Anticuerpos Antivirales Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: Biosens Bioelectron Asunto de la revista: BIOTECNOLOGIA Año: 2014 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Reino Unido