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High prevalence of Candida dubliniensis in lower respiratory tract secretions from cystic fibrosis patients may be related to increased adherence properties.
Wahab, Atqah Abdul; Taj-Aldeen, Saad J; Kolecka, Anna; ElGindi, Mei; Finkel, Jonathan S; Boekhout, Teun.
Afiliación
  • Wahab AA; Departments of Pediatrics, Hamad Medical Corporation, Doha, PO Box 3050, Qatar; Weill Cornell Medical College, Doha, Qatar. Electronic address: atiqaaw@yahoo.com.
  • Taj-Aldeen SJ; Microbiology Division, Laboratory of Medicine and Pathology, Hamad Medical Corporation, Doha, Qatar.
  • Kolecka A; CBS Fungal Biodiversity Center, Utrecht, the Netherlands.
  • ElGindi M; Department of Biological Sciences, Carnegie Mellon University in Qatar, Doha, Qatar.
  • Finkel JS; Department of Biological Sciences, Carnegie Mellon University in Qatar, Doha, Qatar.
  • Boekhout T; CBS Fungal Biodiversity Center, Utrecht, the Netherlands; Department of Internal Medicine and Infectious Diseases, University Medical Center, Utrecht, the Netherlands; Department of Dermatology, Shanghai Key Laboratory of Molecular Medical Mycology, Institute of Dermatology and Medical Mycology, Cha
Int J Infect Dis ; 24: 14-9, 2014 Jul.
Article en En | MEDLINE | ID: mdl-24780917
OBJECTIVES: We identified Candida spp isolated from lower respiratory tract secretions obtained from cystic fibrosis (CF) patients, by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), with the aim of determining the most prevalent causative agent. We also sought to determine their adhesive properties in order to understand their biology related to CF. METHODS: Twenty-five clinical samples were collected from a cohort of 20 CF patients. Twenty-six isolates of Candida spp were isolated and identified by MALDI-TOF MS method. Adherence assays were performed using the Fluxion BioFlux 200, a flow apparatus that allows for the visualization of adhering cells. RESULTS: MALDI-TOF MS analysis revealed C. dubliniensis to be the most prevalent species (n=18, 69%), followed by C. albicans (n=4), C. tropicalis (n=3), and C. glabrata (n=1). C. dubliniensis showed the strongest adherence under constant flow when compared to the other species of Candida. In the majority of cases, C. dubliniensis was isolated in combination with Pseudomonas aeruginosa and Staphylococcus aureus. C. dubliniensis appears to be able to survive in the CF lung and coexist with bacteria. CONCLUSIONS: The data presented here show that the presence of C. dubliniensis in the lower airways of CF patients may be related to increased adherence properties.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones por Pseudomonas / Sistema Respiratorio / Infecciones Estafilocócicas / Candida / Candidiasis / Fibrosis Quística Tipo de estudio: Prevalence_studies / Risk_factors_studies Límite: Adolescent / Adult / Child / Child, preschool / Female / Humans / Infant / Male Idioma: En Revista: Int J Infect Dis Asunto de la revista: DOENCAS TRANSMISSIVEIS Año: 2014 Tipo del documento: Article Pais de publicación: Canadá
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones por Pseudomonas / Sistema Respiratorio / Infecciones Estafilocócicas / Candida / Candidiasis / Fibrosis Quística Tipo de estudio: Prevalence_studies / Risk_factors_studies Límite: Adolescent / Adult / Child / Child, preschool / Female / Humans / Infant / Male Idioma: En Revista: Int J Infect Dis Asunto de la revista: DOENCAS TRANSMISSIVEIS Año: 2014 Tipo del documento: Article Pais de publicación: Canadá