The first electrochemical MIP sensor for tamoxifen.
Sensors (Basel)
; 14(5): 7647-54, 2014 Apr 25.
Article
en En
| MEDLINE
| ID: mdl-24776936
We present an electrochemical MIP sensor for tamoxifen (TAM)-a nonsteroidal anti-estrogen-which is based on the electropolymerisation of an O-phenylenediamineâresorcinol mixture directly on the electrode surface in the presence of the template molecule. Up to now only "bulk" MIPs for TAM have been described in literature, which are applied for separation in chromatography columns. Electro-polymerisation of the monomers in the presence of TAM generated a film which completely suppressed the reduction of ferricyanide. Removal of the template gave a markedly increased ferricyanide signal, which was again suppressed after rebinding as expected for filling of the cavities by target binding. The decrease of the ferricyanide peak of the MIP electrode depended linearly on the TAM concentration between 1 and 100 nM. The TAM-imprinted electrode showed a 2.3 times higher recognition of the template molecule itself as compared to its metabolite 4-hydroxytamoxifen and no cross-reactivity with the anticancer drug doxorubucin was found. Measurements at +1.1 V caused a fouling of the electrode surface, whilst pretreatment of TAM with peroxide in presence of HRP generated an oxidation product which was reducible at 0 mV, thus circumventing the polymer formation and electrochemical interferences.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Polímeros
/
Tamoxifeno
/
Técnicas Biosensibles
/
Conductometría
/
Electrodos
/
Impresión Molecular
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Sensors (Basel)
Año:
2014
Tipo del documento:
Article
País de afiliación:
Alemania
Pais de publicación:
Suiza