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Effects of chebulic acid on advanced glycation endproducts-induced collagen cross-links.
Lee, Ji-Young; Oh, Jun-Gu; Kim, Jin Sook; Lee, Kwang-Won.
Afiliación
  • Lee JY; Department of Food Bioscience and Technology, College of Life Science & Biotechnology, Korea University.
Biol Pharm Bull ; 37(7): 1162-7, 2014.
Article en En | MEDLINE | ID: mdl-24759763
Advanced glycation end-products (AGEs) have been implicated in the development of diabetic complications. We report the antiglycating activity of chebulic acid (CA), isolated from Terminalia chebula on breaking the cross-links of proteins induced by AGEs and inhibiting the formation of AGEs. Aminoguanidine (AG) reduced 50% of glycated bovine serum albumin (BSA) with glycolaldehyde (glycol-BSA)-induced cross-links of collagen at a concentration of 67.8 ± 2.5 mM, the level of CA required for exerting a similar antiglycating activity was 38.8 ± 0.5 µM. Also, the breaking activity on collagen cross-links induced by glycol-BSA was potent with CA (IC50=1.46 ± 0.05 mM), exhibiting 50-fold stronger breaking activity than with ALT-711, a well-known cross-link breaker (IC50=72.2 ± 2.4 mM). IC50 values of DPPH· scavenging activity for CA and ascorbic acid (AA) were 39.2 ± 4.9 and 19.0 ± 1.2 µg dry matter (DM) mL(-1), respectively, and ferric reducing and antioxidant power (FRAP) activities for CA and AA were 4.70 ± 0.06 and 11.4 ± 0.1 mmol/FeSO4·7H2O/g DM, respectively. The chelating activities of CA, AG and ALT711 on copper-catalyzed oxidation of AA were compared, and in increasing order, ALT-711 (IC50 of 1.92 ± 0.20 mM)
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Benzopiranos / Albúmina Sérica Bovina / Depuradores de Radicales Libres / Colágeno / Productos Finales de Glicación Avanzada / Reactivos de Enlaces Cruzados Idioma: En Revista: Biol Pharm Bull Asunto de la revista: BIOQUIMICA / FARMACOLOGIA Año: 2014 Tipo del documento: Article Pais de publicación: Japón
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Benzopiranos / Albúmina Sérica Bovina / Depuradores de Radicales Libres / Colágeno / Productos Finales de Glicación Avanzada / Reactivos de Enlaces Cruzados Idioma: En Revista: Biol Pharm Bull Asunto de la revista: BIOQUIMICA / FARMACOLOGIA Año: 2014 Tipo del documento: Article Pais de publicación: Japón