Orcein-alcian blue staining: a new technique for demonstrating acid mucins in gastrointestinal epithelium.
J Clin Pathol
; 42(8): 881-4, 1989 Aug.
Article
en En
| MEDLINE
| ID: mdl-2475531
Orcein-alcian blue staining, a new method for the simultaneous demonstration of sulphated and sialomucins in gastrointestinal epithelium was compared with the standard high iron diamine-alcian blue technique. Sections were oxidised with potassium permanganate and decolourised in oxalic acid. They were stained with orcein for four hours, differentiated for a few seconds in acid alcohol, and then counterstained with alcian blue for half to one minute. There was a good correlation of results between the two methods. Orcein-alcian blue is a safer, cheaper, and quicker method than high iron diamine-alcian blue which can be safely introduced into routine laboratories for the study of acid mucins in the gastrointestinal diseases.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Oxazinas
/
Coloración y Etiquetado
/
Azul Alcián
/
Mucosa Gástrica
/
Indoles
/
Mucosa Intestinal
/
Mucinas
Límite:
Humans
Idioma:
En
Revista:
J Clin Pathol
Año:
1989
Tipo del documento:
Article
Pais de publicación:
Reino Unido