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Involvement of Neisseria meningitidis lipoprotein GNA2091 in the assembly of a subset of outer membrane proteins.
Bos, Martine P; Grijpstra, Jan; Tommassen-van Boxtel, Ria; Tommassen, Jan.
Afiliación
  • Bos MP; From the Department of Molecular Microbiology, Institute of Biomembranes, Utrecht University, 3584 CH Utrecht, The Netherlands m.bos@uu.nl.
  • Grijpstra J; From the Department of Molecular Microbiology, Institute of Biomembranes, Utrecht University, 3584 CH Utrecht, The Netherlands.
  • Tommassen-van Boxtel R; From the Department of Molecular Microbiology, Institute of Biomembranes, Utrecht University, 3584 CH Utrecht, The Netherlands.
  • Tommassen J; From the Department of Molecular Microbiology, Institute of Biomembranes, Utrecht University, 3584 CH Utrecht, The Netherlands.
J Biol Chem ; 289(22): 15602-10, 2014 May 30.
Article en En | MEDLINE | ID: mdl-24755216
GNA2091 of Neisseria meningitidis is a lipoprotein of unknown function that is included in the novel 4CMenB vaccine. Here, we investigated the biological function and the subcellular localization of the protein. We demonstrate that GNA2091 functions in the assembly of outer membrane proteins (OMPs) because its absence resulted in the accumulation of misassembled OMPs. Cell fractionation and protease accessibility experiments showed that the protein is localized at the periplasmic side of the outer membrane. Pulldown experiments revealed that it is not stably associated with the ß-barrel assembly machinery, the previously identified complex for OMP assembly. Thus, GNA2091 constitutes a novel outer membrane-based lipoprotein required for OMP assembly. Furthermore, its location at the inner side of the outer membrane indicates that protective immunity elicited by this antigen cannot be due to bactericidal or opsonic activity of antibodies.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de la Membrana Bacteriana Externa / Vacunas Bacterianas / Neisseria meningitidis Serogrupo B Idioma: En Revista: J Biol Chem Año: 2014 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de la Membrana Bacteriana Externa / Vacunas Bacterianas / Neisseria meningitidis Serogrupo B Idioma: En Revista: J Biol Chem Año: 2014 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Estados Unidos