Application of a real-time PCR method for Salmonella spp., Escherichia coli, Staphylococcus aureus and Clostridium perfringens detection in water samples.
Pol J Microbiol
; 62(4): 439-43, 2013.
Article
en En
| MEDLINE
| ID: mdl-24730140
The diagnostic assessment of water sanitary state is based mainly on the cultivation of bacteria retained on membrane filters. However classical microbiology methods have a lot of disadvantages. More and more frequently, rapid detection and identification of pathogens present in water is based on molecular biology techniques. The aim of this study was to determine the effectiveness and usefulness of a real-time PCR method, when compared to the recommended bacteria culture method, in diagnostics of pathogens in water samples. The research concerned the detection and identification of main sanitary indicators of water such as: Salmonella spp., Escherichia coli, Staphylococcus aureus and Clostridium perfringens. The analyses were conducted in water samples contaminated with the reference material (the aforementioned bacteria) and real environmental samples, which were examined for the presence of nucleic acid of: Salmonella spp., E. coli, S. aureus and C. perfringens using a real-time PCR method.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Bacterias
/
Microbiología del Agua
/
Reacción en Cadena en Tiempo Real de la Polimerasa
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Pol J Microbiol
Asunto de la revista:
MICROBIOLOGIA
Año:
2013
Tipo del documento:
Article
Pais de publicación:
Polonia