Your browser doesn't support javascript.
loading
Fibrinogen and thrombin concentrations are critical for fibrin glue adherence in rat high-risk colon anastomoses.
Portilla-de Buen, Eliseo; Orozco-Mosqueda, Abel; Leal-Cortés, Caridad; Vázquez-Camacho, Gonzalo; Fuentes-Orozco, Clotilde; Alvarez-Villaseñor, Andrea Socorro; Macías-Amezcua, Michel Dassaejv; González-Ojeda, Alejandro.
Afiliación
  • Portilla-de Buen E; Centro de Investigación Biomédica de Occidente, IMSS, Surgical Research Division, Guadalajara, Jal, México.
  • Orozco-Mosqueda A; Transplantation Department, SSG, Hospital Regional de Alta Especialidad del Bajío, León, Gto, México.
  • Leal-Cortés C; Centro de Investigación Biomédica de Occidente, IMSS, Surgical Research Division, Guadalajara, Jal, México.
  • Vázquez-Camacho G; Cell Biology Laboratory, Instituto Tecnológico de Estudios Superiores de Monterrey, School of Medicine, Campus Guadalajara, Jal, México.
  • Fuentes-Orozco C; Surgical Section of the Research Unit in Clinical Epidemiology, CMNO, IMSS, Hospital de Especialidades, Guadalajara, Jal, México.
  • Alvarez-Villaseñor AS; Medical Care Services, IMSS, Baja California Sur, México.
  • Macías-Amezcua MD; Surgical Section of the Research Unit in Clinical Epidemiology, CMNO, IMSS, Hospital de Especialidades, Guadalajara, Jal, México.
  • González-Ojeda A; Surgical Section of the Research Unit in Clinical Epidemiology, CMNO, IMSS, Hospital de Especialidades, Guadalajara, Jal, México.
Clinics (Sao Paulo) ; 69(4): 259-64, 2014.
Article en En | MEDLINE | ID: mdl-24714834
OBJECTIVE: Fibrin glues have not been consistently successful in preventing the dehiscence of high-risk colonic anastomoses. Fibrinogen and thrombin concentrations in glues determine their ability to function as sealants, healers, and/or adhesives. The objective of the current study was to compare the effects of different concentrations of fibrinogen and thrombin on bursting pressure, leaks, dehiscence, and morphology of high-risk ischemic colonic anastomoses using fibrin glue in rats. METHODS: Colonic anastomoses in adult female Sprague-Dawley rats (weight, 250-350 g) treated with fibrin glue containing different concentrations of fibrinogen and thrombin were evaluated at post-operative day 5. The interventions were low-risk (normal) or high-risk (ischemic) end-to-end colonic anastomoses using polypropylene sutures and topical application of fibrinogen at high (120 mg/mL) or low (40 mg/mL) concentrations and thrombin at high (1000 IU/mL) or low (500 IU/mL) concentrations. RESULTS: Ischemia alone, anastomosis alone, or both together reduced the bursting pressure. Glues containing a low fibrinogen concentration improved this parameter in all cases. High thrombin in combination with low fibrinogen also improved adherence exclusively in low-risk anastomoses. No differences were detected with respect to macroscopic parameters, histopathology, or hydroxyproline content at 5 days post-anastomosis. CONCLUSIONS: Fibrin glue with a low fibrinogen content normalizes the bursting pressure of high-risk ischemic left-colon anastomoses in rats at day 5 after surgery.
Asunto(s)
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Adhesivos Tisulares / Fibrinógeno / Trombina / Adhesivo de Tejido de Fibrina / Colon / Isquemia Tipo de estudio: Etiology_studies / Risk_factors_studies Límite: Animals Idioma: En Revista: Clinics (Sao Paulo) Asunto de la revista: MEDICINA Año: 2014 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Adhesivos Tisulares / Fibrinógeno / Trombina / Adhesivo de Tejido de Fibrina / Colon / Isquemia Tipo de estudio: Etiology_studies / Risk_factors_studies Límite: Animals Idioma: En Revista: Clinics (Sao Paulo) Asunto de la revista: MEDICINA Año: 2014 Tipo del documento: Article Pais de publicación: Estados Unidos