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A simian-human immunodeficiency virus carrying the rt gene from Chinese CRF01_AE strain of HIV is sensitive to nucleoside reverse transcriptase inhibitors and has a highly genetic stability in vivo.
Wang, Wei; Yao, Nan; Ju, Bin; Dong, Zhihui; Cong, Zhe; Jiang, Hong; Qin, Chuan; Wei, Qiang.
Afiliación
  • Wang W; Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health, Beijing 100021, PR China; Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine, Beijing 100021, PR China; Institute of Laboratory Animal Science, Chinese Academy of Medical Scie
  • Yao N; Comparative Medical Center, Peking Union Medical College, No. 5 Panjiayuan Nanli, Chaoyang District, Beijing 100021, PR China.
  • Ju B; Comparative Medical Center, Peking Union Medical College, No. 5 Panjiayuan Nanli, Chaoyang District, Beijing 100021, PR China.
  • Dong Z; Comparative Medical Center, Peking Union Medical College, No. 5 Panjiayuan Nanli, Chaoyang District, Beijing 100021, PR China.
  • Cong Z; Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health, Beijing 100021, PR China; Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine, Beijing 100021, PR China; Institute of Laboratory Animal Science, Chinese Academy of Medical Scie
  • Jiang H; Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health, Beijing 100021, PR China; Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine, Beijing 100021, PR China; Institute of Laboratory Animal Science, Chinese Academy of Medical Scie
  • Qin C; Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health, Beijing 100021, PR China; Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine, Beijing 100021, PR China; Institute of Laboratory Animal Science, Chinese Academy of Medical Scie
  • Wei Q; Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health, Beijing 100021, PR China; Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine, Beijing 100021, PR China; Institute of Laboratory Animal Science, Chinese Academy of Medical Scie
Microbes Infect ; 16(6): 461-71, 2014 Jun.
Article en En | MEDLINE | ID: mdl-24709063
Human immunodeficiency virus (HIV)-1 subtype CRF01_AE is one of the major HIV-1 subtypes that dominate the global epidemic. However, its drug resistance, associated mutations, and viral fitness have not been systemically studied, because available chimeric simian-HIVs (SHIVs) usually express the HIV-1 reverse transcriptase (rt) gene of subtype B HIV-1, which is different from subtype CRF01_AE HIV-1. In this study, a recombinant plasmid, pRT-SHIV/AE, was constructed to generate a chimeric RT-SHIV/AE by replacing the rt gene of simian immunodeficiency virus (SIVmac239) with the counterpart of Chinese HIV-1 subtype CRF01_AE. The infectivity, replication capacity, co-receptor tropism, drug sensitivity, and genetic stability of RT-SHIV/AE were characterized. The new chimeric RT-SHIV/AE effectively infected and replicated in human T cell line and rhesus peripheral blood mononuclear cells (rhPBMC). The rt gene of RT-SHIV/AE lacked the common mutation (T215I) associated with drug resistance. RT-SHIV-AE retained infectivity and immunogenicity, similar to that of its counterpart RT-SHIV/TC virus following intravenous inoculation in Chinese rhesus macaque. RT-SHIV-AE was more sensitive to nucleoside reverse transcriptase inhibitors (NRTIs) than the RT-SHIV/TC. RT-SHIV/AE was genetically stable in Chinese rhesus macaque. The new chimeric RT-SHIV/AE may be a valuable tool for evaluating the efficacy of the rt-based antiviral drugs against the subtype CRF01_AE HIV-1.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: VIH / Virus de la Inmunodeficiencia de los Simios / Virus Reordenados / Transcriptasa Inversa del VIH Tipo de estudio: Diagnostic_studies Límite: Animals / Humans País/Región como asunto: Asia Idioma: En Revista: Microbes Infect Asunto de la revista: ALERGIA E IMUNOLOGIA / MICROBIOLOGIA Año: 2014 Tipo del documento: Article Pais de publicación: Francia
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: VIH / Virus de la Inmunodeficiencia de los Simios / Virus Reordenados / Transcriptasa Inversa del VIH Tipo de estudio: Diagnostic_studies Límite: Animals / Humans País/Región como asunto: Asia Idioma: En Revista: Microbes Infect Asunto de la revista: ALERGIA E IMUNOLOGIA / MICROBIOLOGIA Año: 2014 Tipo del documento: Article Pais de publicación: Francia