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Hsp90 blockers inhibit adipocyte differentiation and fat mass accumulation.
Desarzens, Sébastien; Liao, Wan-Hui; Mammi, Caterina; Caprio, Massimiliano; Faresse, Nourdine.
Afiliación
  • Desarzens S; University of Zurich, Zurich, Switzerland.
  • Liao WH; University of Zurich, Zurich, Switzerland.
  • Mammi C; IRCCS San Raffaele, Roma, Italy.
  • Caprio M; IRCCS San Raffaele, Roma, Italy.
  • Faresse N; University of Zurich, Zurich, Switzerland.
PLoS One ; 9(4): e94127, 2014.
Article en En | MEDLINE | ID: mdl-24705830
Geldanamycin derivatives are benzoquinone ansamycin antibiotics that bind to Hsp90 and alter its function. The alteration of Hsp90 activity limits some cellular hormonal responses by inhibiting nuclear receptors activation. The nuclear receptors activity, such as PPARγ, the mineralocorticoid and glucocorticoid receptors (MR and GR) play a critical role in the conversion of preadipocytes to mature adipocytes. Given the importance of these nuclear receptors for adipogenesis, we investigated the effects of geldanamycin analogues (GA) on adipocyte differentiation and function. We found that early exposure of preadipocyte cells to GA inhibited their conversion into mature adipocytes by inhibiting the adipogenic transcriptional program and lipid droplets accumulation. Furthermore, GA altered the adipokines secretion profile of mature adipocyte. The anti-adipogenic effect of GA was also confirmed in mice fed a high fat diet. Biochemical analysis revealed that anti-adipogenic effects of geldanamycin analogues may result from the simultaneous inhibition of MR, GR and PPARγ activity. Taken together, our observations lead us to propose Hsp90 as a potent target for drug development in the control of obesity and its related metabolic complications.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Diferenciación Celular / Adipocitos / Proteínas HSP90 de Choque Térmico Límite: Animals Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2014 Tipo del documento: Article País de afiliación: Suiza Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Diferenciación Celular / Adipocitos / Proteínas HSP90 de Choque Térmico Límite: Animals Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2014 Tipo del documento: Article País de afiliación: Suiza Pais de publicación: Estados Unidos