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Clavulanic acid production by the MMS 150 mutant obtained from wild type Streptomyces clavuligerus ATCC 27064.
da Silva Vasconcelos, Eliton; de Lima, Vanderlei Aparecido; Goto, Leandro Seiji; Cruz-Hernández, Isara Lourdes; Hokka, Carlos Osamu.
Afiliación
  • da Silva Vasconcelos E; Departamento de Engenharia Química, Universidade Federal de São Carlos, São Carlos SP, Brazil.
  • de Lima VA; Departamento de Engenharia Química, Universidade Federal de São Carlos, São Carlos SP, Brazil.
  • Goto LS; Departamento de Engenharia Química, Universidade Federal de São Carlos, São Carlos SP, Brazil. ; Grupo de Biofísica Molecular "Sérgio Mascarenhas", Instituto de Física de São Carlos, Universidade de São Paulo, São Carlos, SP, Brazil.
  • Cruz-Hernández IL; Departamento de Engenharia Química, Universidade Federal de São Carlos, São Carlos SP, Brazil.
  • Hokka CO; Departamento de Engenharia Química, Universidade Federal de São Carlos, São Carlos SP, Brazil.
Braz J Microbiol ; 44(4): 1049-57, 2013 Dec.
Article en En | MEDLINE | ID: mdl-24688492
Clavulanic acid (CA) is a powerful inhibitor of the beta-lactamases, enzymes produced by bacteria resistants to penicillin and cefalosporin. This molecule is produced industrially by strains of Streptomyces clavuligerus in complex media which carbon and nitrogen resources are supplied by inexpensive compounds still providing high productivity. The genetic production improvement using physical and chemical mutagenic agents is an important strategy in programs of industrial production development of bioactive metabolites. However, parental strains are susceptible to loss of their original productivity due genetic instability phenomenona. In this work, some S. clavuligerus mutant strains obtained by treatment with UV light and with MMS are compared with the wild type (Streptomyces clavuligerus ATCC 27064). The results indicated that the random mutations originated some strains with different phenotypes, most divergent demonstrated by the mutants strains named AC116, MMS 150 and MMS 54, that exhibited lack of pigmentation in their mature spores. Also, the strain MMS 150 presented a larger production of CA when cultivated in semi-synthetics media. Using other media, the wild type strain obtained a larger CA production. Besides, using the modifed complex media the MMS 150 strain showed changes in its lipolitic activity and a larger production of CA. The studies also allowed finding the best conditions for a lipase activity exhibited by wild type S. clavuligerus and the MMS150 mutant.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Streptomyces / Mutagénesis / Ácido Clavulánico / Ingeniería Metabólica / Mutación Idioma: En Revista: Braz J Microbiol Año: 2013 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Brasil
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Streptomyces / Mutagénesis / Ácido Clavulánico / Ingeniería Metabólica / Mutación Idioma: En Revista: Braz J Microbiol Año: 2013 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Brasil