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Reduced hTERT protein levels are associated with DNA aneuploidy in the colonic mucosa of patients suffering from longstanding ulcerative colitis.
Friis-Ottessen, Mariann; De Angelis, Paula M; Schjølberg, Aasa R; Andersen, Solveig N; Clausen, Ole Petter F.
Afiliación
  • Friis-Ottessen M; Division of Diagnostics and Intervention, Department of Pathology, Oslo University Hospital, Rikshospitalet, 0424 Oslo, Norway.
  • De Angelis PM; Division of Diagnostics and Intervention, Department of Pathology, Oslo University Hospital, Rikshospitalet, 0424 Oslo, Norway.
  • Schjølberg AR; Department of Pathology, University of Oslo, 0424 Oslo, Norway.
  • Andersen SN; Department of Pathology, Akershus University Hospital, Division of Medicine and Laboratory Sciences, University of Oslo, 1474 Nordbyhagen, Norway.
  • Clausen OP; Division of Diagnostics and Intervention, Department of Pathology, Oslo University Hospital, Rikshospitalet, 0424 Oslo, Norway.
Int J Mol Med ; 33(6): 1477-83, 2014 Jun.
Article en En | MEDLINE | ID: mdl-24676865
Longstanding ulcerative colitis (UC) is a disease of chronic inflammation of the colon. It is associated with the development of colorectal cancer through a multistep process including increasing degrees of dysplasia and DNA-ploidy changes. However, not all UC patients will develop these characteristics even during lifelong disease, and patients may therefore be divided into progressors who develop dysplasia or cancer, and non-progressors who do not exhibit such changes. In the present study, the amount of hTERT, the catalytic subunit of the enzyme telomerase, was estimated by using peroxidase immunohistochemistry (IHC) in a set of progressor and non-progressor UC colectomies. The protein levels in the colonic mucosa of the progressors and non­progressors were compared, and further comparisons between different categories of dysplastic development and to DNA-ploidy status within the progressors were made. Levels of hTERT were elevated in the colonic mucosa of the progressors and non-progressors when compared to non-UC control samples, but no difference was observed between the hTERT levels in the mucosa of progressors and non-progressors. The levels of hTERT associated with levels of Ki67 to a significant degree within the non-progressors. hTERT expression in lesions with DNA-aneuploidy were decreased as compared to diploid lesions, when stratified for different classes of colonic morphology. Our results indicate an association between hTERT protein expression and aneuploidy in UC-progressor colons, and also a possible protective mechanism in the association between hTERT and Ki67, against development of malignant features within the mucosa of a UC-colon.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Colitis Ulcerosa / Colon / Telomerasa / Mucosa Intestinal Tipo de estudio: Risk_factors_studies Límite: Adolescent / Adult / Child / Female / Humans / Male / Middle aged Idioma: En Revista: Int J Mol Med Asunto de la revista: BIOLOGIA MOLECULAR / GENETICA MEDICA Año: 2014 Tipo del documento: Article País de afiliación: Noruega Pais de publicación: Grecia
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Colitis Ulcerosa / Colon / Telomerasa / Mucosa Intestinal Tipo de estudio: Risk_factors_studies Límite: Adolescent / Adult / Child / Female / Humans / Male / Middle aged Idioma: En Revista: Int J Mol Med Asunto de la revista: BIOLOGIA MOLECULAR / GENETICA MEDICA Año: 2014 Tipo del documento: Article País de afiliación: Noruega Pais de publicación: Grecia