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MicroRNA319 positively regulates cold tolerance by targeting OsPCF6 and OsTCP21 in rice (Oryza sativa L.).
Wang, Sun-ting; Sun, Xiao-li; Hoshino, Yoichiro; Yu, Yang; Jia, Bei; Sun, Zhong-wen; Sun, Ming-zhe; Duan, Xiang-bo; Zhu, Yan-ming.
Afiliación
  • Wang ST; Key Laboratory of Agricultural Biological Functional Genes, Northeast Agricultural University, Harbin, P.R. China.
  • Sun XL; Key Laboratory of Agricultural Biological Functional Genes, Northeast Agricultural University, Harbin, P.R. China.
  • Hoshino Y; Field Science Center for Northern Biosphere, Hokkaido University, Kita-ku, Sapporo, Hokkaido, Japan.
  • Yu Y; Key Laboratory of Agricultural Biological Functional Genes, Northeast Agricultural University, Harbin, P.R. China.
  • Jia B; Key Laboratory of Agricultural Biological Functional Genes, Northeast Agricultural University, Harbin, P.R. China.
  • Sun ZW; Key Laboratory of Agricultural Biological Functional Genes, Northeast Agricultural University, Harbin, P.R. China.
  • Sun MZ; Key Laboratory of Agricultural Biological Functional Genes, Northeast Agricultural University, Harbin, P.R. China.
  • Duan XB; Key Laboratory of Agricultural Biological Functional Genes, Northeast Agricultural University, Harbin, P.R. China.
  • Zhu YM; Key Laboratory of Agricultural Biological Functional Genes, Northeast Agricultural University, Harbin, P.R. China.
PLoS One ; 9(3): e91357, 2014.
Article en En | MEDLINE | ID: mdl-24667308
The microRNA319 (miR319) family is conserved among diverse plant species. In rice (Oryza sativa L.), the miR319 gene family is comprised of two members, Osa-miR319a and Osa-miR319b. We found that overexpressing Osa-miR319b in rice resulted in wider leaf blades and delayed development. Here, we focused on the biological function and potential molecular mechanism of the Osa-miR319b gene in response to cold stress in rice. The expression of Osa-miR319b was down-regulated by cold stress, and the overexpression of Osa-miR319b led to an enhanced tolerance to cold stress, as evidenced by higher survival rates and proline content. Also, the expression of a handful of cold stress responsive genes, such as DREB1A/B/C, DREB2A, TPP1/2, was increased in Osa-miR319b transgenic lines. Furthermore, we demonstrated the nuclear localization of the transcription factors, OsPCF6 and OsTCP21, which may be Osa-miR319b-targeted genes. We also showed that OsPCF6 and OsTCP21 expression was largely induced by cold stress, and the degree of induction was obviously repressed in plants overexpressing Osa-miR319b. As expected, the down-regulation of OsPCF6 and OsTCP21 resulted in enhanced tolerance to cold stress, partially by modifying active oxygen scavenging. Taken together, our findings suggest that Osa-miR319b plays an important role in plant response to cold stress, maybe by targeting OsPCF6 and OsTCP21.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oryza / Estrés Fisiológico / Adaptación Fisiológica / Genes de Plantas / Hojas de la Planta / Regulación de la Expresión Génica de las Plantas / MicroARNs Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2014 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oryza / Estrés Fisiológico / Adaptación Fisiológica / Genes de Plantas / Hojas de la Planta / Regulación de la Expresión Génica de las Plantas / MicroARNs Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2014 Tipo del documento: Article Pais de publicación: Estados Unidos