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Noninvasive fetal RHD genotyping from maternal plasma in an admixed Brazilian population.
Schmidt, L C; Cabral, A C V; Faria, M A; Monken, F; Tarazona-Santos, E; Martins, M L.
Afiliación
  • Schmidt LC; Departamento de Imunohematologia, Fundação Hemominas, Belo Horizonte, MG, Brasil luciana.cayres@hemominas.mg.gov.br.
  • Cabral AC; Centro de Medicina Fetal, Departamento de Obstetrícia e Ginecologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.
  • Faria MA; Departamento de Pesquisa, Fundação Hemominas, Belo Horizonte, MG, Brasil.
  • Monken F; Centro de Medicina Fetal, Departamento de Obstetrícia e Ginecologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.
  • Tarazona-Santos E; Departamento de Biologia Geral, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.
  • Martins ML; Departamento de Pesquisa, Fundação Hemominas, Belo Horizonte, MG, Brasil.
Genet Mol Res ; 13(1): 799-805, 2014 Feb 07.
Article en En | MEDLINE | ID: mdl-24615044
We evaluated the efficacy of noninvasive fetal Rhesus D (RHD) genotyping from maternal plasma in a highly admixed population. Fifty-five blood samples from RhD-negative pregnant women from Brazil were processed for extraction of cell-free plasma DNA. Real-time PCR was performed to amplify segments of exons 5 and 7 from the RHD gene, as well as for detection of the SRY gene to confirm the presence of fetal DNA. Fetal genotyping results were compared with the RhD phenotype determined from newborn cord blood samples obtained at birth. Thirty-two samples were RHD-positive, 18 were RHD-negative and 5 were inconclusive due to amplification of only one RHD exon. In 43 samples, the fetal RHD genotype was compared to the neonatal RhD phenotype, and only one result was discordant, due to false-negative serology. There was one false SRY genotyping negative result. We conclude that noninvasive fetal RHD genotyping from maternal blood provides accurate results and suggests its viability as a clinical tool for the management of RhD-negative pregnant women in an admixed population.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Diagnóstico Prenatal / Sistema del Grupo Sanguíneo Rh-Hr Tipo de estudio: Diagnostic_studies Límite: Female / Humans / Newborn / Pregnancy País/Región como asunto: America do sul / Brasil Idioma: En Revista: Genet Mol Res Asunto de la revista: BIOLOGIA MOLECULAR / GENETICA Año: 2014 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Brasil
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Diagnóstico Prenatal / Sistema del Grupo Sanguíneo Rh-Hr Tipo de estudio: Diagnostic_studies Límite: Female / Humans / Newborn / Pregnancy País/Región como asunto: America do sul / Brasil Idioma: En Revista: Genet Mol Res Asunto de la revista: BIOLOGIA MOLECULAR / GENETICA Año: 2014 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Brasil