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Photodynamic antibacterial effect of graphene quantum dots.
Ristic, Biljana Z; Milenkovic, Marina M; Dakic, Ivana R; Todorovic-Markovic, Biljana M; Milosavljevic, Momir S; Budimir, Milica D; Paunovic, Verica G; Dramicanin, Miroslav D; Markovic, Zoran M; Trajkovic, Vladimir S.
Afiliación
  • Ristic BZ; Institute of Microbiology and Immunology, School of Medicine, University of Belgrade, Dr. Subotica 1, 11000 Belgrade, Serbia.
  • Milenkovic MM; Institute of Microbiology and Immunology, School of Medicine, University of Belgrade, Dr. Subotica 1, 11000 Belgrade, Serbia.
  • Dakic IR; Institute of Microbiology and Immunology, School of Medicine, University of Belgrade, Dr. Subotica 1, 11000 Belgrade, Serbia.
  • Todorovic-Markovic BM; Vinca Institute of Nuclear Sciences, University of Belgrade, 11000 Belgrade, Serbia.
  • Milosavljevic MS; Vinca Institute of Nuclear Sciences, University of Belgrade, 11000 Belgrade, Serbia.
  • Budimir MD; Vinca Institute of Nuclear Sciences, University of Belgrade, 11000 Belgrade, Serbia.
  • Paunovic VG; Institute of Microbiology and Immunology, School of Medicine, University of Belgrade, Dr. Subotica 1, 11000 Belgrade, Serbia.
  • Dramicanin MD; Institute of Microbiology and Immunology, School of Medicine, University of Belgrade, Dr. Subotica 1, 11000 Belgrade, Serbia.
  • Markovic ZM; Vinca Institute of Nuclear Sciences, University of Belgrade, 11000 Belgrade, Serbia. Electronic address: zormark@vinca.rs.
  • Trajkovic VS; Institute of Microbiology and Immunology, School of Medicine, University of Belgrade, Dr. Subotica 1, 11000 Belgrade, Serbia. Electronic address: vtrajkovic@med.bg.ac.rs.
Biomaterials ; 35(15): 4428-35, 2014 May.
Article en En | MEDLINE | ID: mdl-24612819
Synthesis of new antibacterial agents is becoming increasingly important in light of the emerging antibiotic resistance. In the present study we report that electrochemically produced graphene quantum dots (GQD), a new class of carbon nanoparticles, generate reactive oxygen species when photoexcited (470 nm, 1 W), and kill two strains of pathogenic bacteria, methicillin-resistant Staphylococcus aureus and Escherichia coli. Bacterial killing was demonstrated by the reduction in number of bacterial colonies in a standard plate count method, the increase in propidium iodide uptake confirming the cell membrane damage, as well as by morphological defects visualized by atomic force microscopy. The induction of oxidative stress in bacteria exposed to photoexcited GQD was confirmed by staining with a redox-sensitive fluorochrome dihydrorhodamine 123. Neither GQD nor light exposure alone were able to cause oxidative stress and reduce the viability of bacteria. Importantly, mouse spleen cells were markedly less sensitive in the same experimental conditions, thus indicating a fairly selective antibacterial photodynamic action of GQD.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fármacos Fotosensibilizantes / Puntos Cuánticos / Escherichia coli / Staphylococcus aureus Resistente a Meticilina / Grafito / Antibacterianos Límite: Animals / Humans Idioma: En Revista: Biomaterials Año: 2014 Tipo del documento: Article Pais de publicación: Países Bajos
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fármacos Fotosensibilizantes / Puntos Cuánticos / Escherichia coli / Staphylococcus aureus Resistente a Meticilina / Grafito / Antibacterianos Límite: Animals / Humans Idioma: En Revista: Biomaterials Año: 2014 Tipo del documento: Article Pais de publicación: Países Bajos