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Store-operated Ca2+ entry (SOCE) regulates melanoma proliferation and cell migration.
Umemura, Masanari; Baljinnyam, Erdene; Feske, Stefan; De Lorenzo, Mariana S; Xie, Lai-Hua; Feng, Xianfeng; Oda, Kayoko; Makino, Ayako; Fujita, Takayuki; Yokoyama, Utako; Iwatsubo, Mizuka; Chen, Suzie; Goydos, James S; Ishikawa, Yoshihiro; Iwatsubo, Kousaku.
Afiliación
  • Umemura M; Cardiovascular Research Institute, Yokohama City University School of Medicine, Yokohama, Japan.
  • Baljinnyam E; Department of Cell Biology and Molecular Medicine, New Jersey Medical School, Rutgers, The State University of New Jersey, Newark, New Jersey, United States of America.
  • Feske S; Department of Pathology, New York University School of Medicine, New York, New York, United States of America.
  • De Lorenzo MS; Department of Cell Biology and Molecular Medicine, New Jersey Medical School, Rutgers, The State University of New Jersey, Newark, New Jersey, United States of America.
  • Xie LH; Department of Cell Biology and Molecular Medicine, New Jersey Medical School, Rutgers, The State University of New Jersey, Newark, New Jersey, United States of America.
  • Feng X; Cardiovascular Research Institute, Yokohama City University School of Medicine, Yokohama, Japan.
  • Oda K; Cardiovascular Research Institute, Yokohama City University School of Medicine, Yokohama, Japan.
  • Makino A; Cardiovascular Research Institute, Yokohama City University School of Medicine, Yokohama, Japan.
  • Fujita T; Cardiovascular Research Institute, Yokohama City University School of Medicine, Yokohama, Japan.
  • Yokoyama U; Cardiovascular Research Institute, Yokohama City University School of Medicine, Yokohama, Japan.
  • Iwatsubo M; Department of Cell Biology and Molecular Medicine, New Jersey Medical School, Rutgers, The State University of New Jersey, Newark, New Jersey, United States of America.
  • Chen S; Department of Chemical Biology, Susan Lehman Cullen Laboratory of Cancer Research in the Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, Piscataway, New Jersey, United States of America.
  • Goydos JS; Division of Surgical Oncology, Department of Surgery, Robert Wood Johnson Medical School, Rutgers, The State University of New Jersey, Piscataway, New Jersey, United States of America.
  • Ishikawa Y; Cardiovascular Research Institute, Yokohama City University School of Medicine, Yokohama, Japan.
  • Iwatsubo K; Cardiovascular Research Institute, Yokohama City University School of Medicine, Yokohama, Japan ; Department of Cell Biology and Molecular Medicine, New Jersey Medical School, Rutgers, The State University of New Jersey, Newark, New Jersey, United States of America.
PLoS One ; 9(2): e89292, 2014.
Article en En | MEDLINE | ID: mdl-24586666
Store-operated Ca(2+) entry (SOCE) is a major mechanism of Ca(2) (+) import from extracellular to intracellular space, involving detection of Ca(2+) store depletion in endoplasmic reticulum (ER) by stromal interaction molecule (STIM) proteins, which then translocate to plasma membrane and activate Orai Ca(2+) channels there. We found that STIM1 and Orai1 isoforms were abundantly expressed in human melanoma tissues and multiple melanoma/melanocyte cell lines. We confirmed that these cell lines exhibited SOCE, which was inhibited by knockdown of STIM1 or Orai1, or by a pharmacological SOCE inhibitor. Inhibition of SOCE suppressed melanoma cell proliferation and migration/metastasis. Induction of SOCE was associated with activation of extracellular-signal-regulated kinase (ERK), and was inhibited by inhibitors of calmodulin kinase II (CaMKII) or Raf-1, suggesting that SOCE-mediated cellular functions are controlled via the CaMKII/Raf-1/ERK signaling pathway. Our findings indicate that SOCE contributes to melanoma progression, and therefore may be a new potential target for treatment of melanoma, irrespective of whether or not Braf mutation is present.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Cutáneas / Movimiento Celular / Calcio / Señalización del Calcio / Proliferación Celular / Melanocitos / Melanoma Límite: Animals / Humans Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2014 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Cutáneas / Movimiento Celular / Calcio / Señalización del Calcio / Proliferación Celular / Melanocitos / Melanoma Límite: Animals / Humans Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2014 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos