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The immunomodulatory and anti-apoptotic effect of dexamethasone in imminent preterm labor: an experimental study.
Makrydima, Sofia F; Pistiki, Aikaterini C; Chrelias, Charalampos G; Sioulas, Vasileios D; Siristatidis, Charalampos S; Giamarellos-Bourboulis, Evangelos J; Kassanos, Demetrios P.
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  • Makrydima SF; 3rd Department of Obstetrics and Gynecology, Attikon University Hospital, 12462 Athens, Greece. Electronic address: soma20med@hotmail.com.
  • Pistiki AC; 4th Department of Internal Medicine, Attikon University Hospital, 12462 Athens, Greece.
  • Chrelias CG; 3rd Department of Obstetrics and Gynecology, Attikon University Hospital, 12462 Athens, Greece.
  • Sioulas VD; 3rd Department of Obstetrics and Gynecology, Attikon University Hospital, 12462 Athens, Greece.
  • Siristatidis CS; 3rd Department of Obstetrics and Gynecology, Attikon University Hospital, 12462 Athens, Greece.
  • Giamarellos-Bourboulis EJ; Center for Sepsis Control and Care, Jena University Hospital, 07747 Jena, Germany; 4th Department of Internal Medicine, Attikon University Hospital, 12462 Athens, Greece.
  • Kassanos DP; 3rd Department of Obstetrics and Gynecology, Attikon University Hospital, 12462 Athens, Greece.
Eur J Pharmacol ; 730: 31-5, 2014 May 05.
Article en En | MEDLINE | ID: mdl-24582761
The study was designed to investigate the effect of dexamethasone (DEX) on the latency period to delivery in a murine model of preterm labor. To this purpose, pregnant mice were randomly assigned in groups: the control group received water for injection (n=20), the preterm labor group was injected with lipopolysaccharide (LPS) (n=22), while the glucocorticoids group was administered DEX either 1h before (n=17) or after (n=7) lipopolysaccharide. In a first set of experiments animals were monitored to record perinatal outcomes. In another set of experiments, the remaining animals were sacrificed eight h after interventions. Fetuses were homogenized to measure tumor necrosis alpha in supernatants. Maternal splenocytes were isolated and stimulated for cytokine production. Serum of mice was incubated with donor cells from healthy pregnant and non-pregnant animals to induce apoptosis. LPS induced preterm labor but treatment or pretreatment with DEX delayed parturition exerting a favorable impact on survival of delivered fetuses. DEX inverted the increase of fetoplacental tumor necrosis alpha levels. Serum of LPS-stimulated mice induced apoptosis of splenocytes of either pregnant or non-pregnant healthy mice; this was reversed after incubation of splenocytes with serum coming from DEX pre-treated mice. The presented findings suggest that DEX administered either as pre-treatment or treatment prolonged gestation and promoted neonatal survival in a sterile murine model of preterm labor. These favorable outcomes were closely linked to alterations in both immune and apoptotic responses of animals.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Dexametasona / Apoptosis / Factores Inmunológicos / Trabajo de Parto Prematuro Tipo de estudio: Prognostic_studies Límite: Animals / Pregnancy Idioma: En Revista: Eur J Pharmacol Año: 2014 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Dexametasona / Apoptosis / Factores Inmunológicos / Trabajo de Parto Prematuro Tipo de estudio: Prognostic_studies Límite: Animals / Pregnancy Idioma: En Revista: Eur J Pharmacol Año: 2014 Tipo del documento: Article Pais de publicación: Países Bajos