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Detection of monoclonal IGH rearrangements in circulating cells from healthy first-degree relatives of patients with multiple myeloma.
García-Castillo, Herbert; Leal-Ugarte, Evelia; Ortiz Lazareno, Pablo César; Barrera-Chairez, Esperanza; Rosales-García, Víctor Hugo; Barros-Núñez, Patricio.
Afiliación
  • García-Castillo H; División de Genética, Centro de Investigación de Occidente, Instituto Mexicano del Seguro Social, Guadalajara, Jalisco, Mexico.
Med Oncol ; 31(4): 900, 2014 Apr.
Article en En | MEDLINE | ID: mdl-24577939
Multiple myeloma (MM) is characterized by abnormal proliferation of clonal plasma cells or monoclonal plasmacytosis, resulting in accumulation of clonal immunoglobulins. Monoclonal gammopathy of unknown significance (MGUS) is considered a premorbid stage for developing MM. Studies have shown an increased risk of MGUS in first-degree relatives of patients with MM. Detection of immunoglobulin heavy chain gene (IGH) rearrangement provides a useful tool for assessing clonality. The aim of this study was to determine clonality in peripheral blood samples from 61 healthy first-degree relatives of MM probands by sorting circulating lymphocytes and detection of the IGH rearrangements in these cells. We detected 16 out of 61 (26.2%) relatives with monoclonal complete and incomplete IGH rearrangements; only three of them showed elevated monoclonal immunoglobulin in the serum protein electrophoresis. We conclude that this strategy is able to identify efficiently clonality in peripheral blood samples from first-degree relatives of patients with MM, who have a non-negligible risk of developing MGUS or other plasma cell dyscrasias.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Reordenamiento Génico / Cadenas Pesadas de Inmunoglobulina / Mieloma Múltiple Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Adolescent / Adult / Aged80 / Female / Humans / Male / Middle aged Idioma: En Revista: Med Oncol Asunto de la revista: NEOPLASIAS Año: 2014 Tipo del documento: Article País de afiliación: México Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Reordenamiento Génico / Cadenas Pesadas de Inmunoglobulina / Mieloma Múltiple Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Adolescent / Adult / Aged80 / Female / Humans / Male / Middle aged Idioma: En Revista: Med Oncol Asunto de la revista: NEOPLASIAS Año: 2014 Tipo del documento: Article País de afiliación: México Pais de publicación: Estados Unidos