Your browser doesn't support javascript.
loading
Beta-lactamic resistance profiles in Porphyromonas, Prevotella, and Parvimonas species isolated from acute endodontic infections.
Montagner, Francisco; Jacinto, Rogério Castilho; Correa Signoretti, Fernanda Graziela; Scheffer de Mattos, Vanessa; Grecca, Fabiana Soares; Gomes, Brenda Paula Figueiredo de Almeida.
Afiliación
  • Montagner F; Endodontic Division, Department of Conservative Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil. Electronic address: francisco.montagner@ufrgs.br.
  • Jacinto RC; Endodontic Division, Department of Semiology and Clinics, Federal University of Pelotas, Pelotas, Rio Grande do Sul, Brazil.
  • Correa Signoretti FG; Endodontic Division, Piracicaba Dental School, University of Campinas, Piracicaba, São Paulo, Brazil.
  • Scheffer de Mattos V; Endodontic Division, Department of Conservative Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.
  • Grecca FS; Endodontic Division, Department of Conservative Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.
  • Gomes BP; Endodontic Division, Piracicaba Dental School, University of Campinas, Piracicaba, São Paulo, Brazil.
J Endod ; 40(3): 339-44, 2014 Mar.
Article en En | MEDLINE | ID: mdl-24565649
INTRODUCTION: Susceptibility to beta-lactamic agents has changed among anaerobic isolates from acute endodontic infections. The aim of the present study was to determine the prevalence of the cfxA/cfxA2 gene in Prevotella spp., Porphyromonas spp., and Parviomonas micra strains and show its phenotypic expression. METHODS: Root canal samples from teeth with acute endodontic infections were collected and Porphyromonas, Prevotella, and Parvimonas micra strains were isolated and microbiologically identified with conventional culture techniques. The susceptibility of the isolates was determined by the minimum inhibitory concentration of benzylpenicillin, amoxicillin, and amoxicillin + clavulanate using the E-test method (AB BIODISK, Solna, Sweden). The presence of the cfxA/cfxA2 gene was determined through primer-specific polymerase chain reaction. The nitrocefin test was used to determine the expression of the lactamase enzyme. RESULTS: Prevotella disiens, Prevotella oralis, Porphyromonas gingivalis, and P. micra strains were susceptible to benzylpenicillin, amoxicillin, and amoxicillin + clavulanate. The cfxA/cfxA2 gene was detected in 2 of 29 isolates (6.9%). Simultaneous detection of the cfxA/cfxA2 gene and lactamase production was observed for 1 Prevotella buccalis strain. The gene was in 1 P. micra strain but was not expressed. Three strains were positive for lactamase production, but the cfxA/cfxA2 gene was not detected through polymerase chain reaction. CONCLUSIONS: There is a low prevalence of the cfxA/cfxA2 gene and its expression in Porphyromonas spp., Prevotella spp., and P. micra strains isolated from acute endodontic infections. Genetic and phenotypic screening must be performed simultaneously to best describe additional mechanisms involved in lactamic resistance for strict anaerobes.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Peptostreptococcus / Porphyromonas / Prevotella / Resistencia betalactámica / Enfermedades de la Pulpa Dental Tipo de estudio: Risk_factors_studies Límite: Humans Idioma: En Revista: J Endod Año: 2014 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Peptostreptococcus / Porphyromonas / Prevotella / Resistencia betalactámica / Enfermedades de la Pulpa Dental Tipo de estudio: Risk_factors_studies Límite: Humans Idioma: En Revista: J Endod Año: 2014 Tipo del documento: Article Pais de publicación: Estados Unidos