The development of transgenic mice for the expression of large amounts of human lysozyme in milk.
Biotechnol Lett
; 36(6): 1197-202, 2014 Jun.
Article
en En
| MEDLINE
| ID: mdl-24563307
Human lysozyme (hLYZ) has important potential applications as antimicrobial medicine and food additive. To develop a robust expression vector that ensures expression of large amounts of hLYZ in milk, here a 26,267 bp chimeric mouse whey acidic protein (mWAP)::hLYZ cassette was constructed and used as a mammary gland-specific expression vector, in which a 3,010 bp genomic sequence in the 24,466 bp mWAP gene locus was substituted by a 4,811 bp genomic sequence of hLYZ, exactly from the start codon to the stop codon. Corresponding transgenic mice were generated, and enzymatically-active hLYZ was expressed at 18.4-35 g l(-1) in the milk of most transgenic mouse lines. Our transgenic mice carrying chimeric mWAP::hLYZ represent a model system for cost-effective production of hLYZ.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Muramidasa
/
Leche Humana
/
Proteínas de la Leche
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Biotechnol Lett
Año:
2014
Tipo del documento:
Article
Pais de publicación:
Países Bajos