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Prospects for lentiviral vector mediated prostaglandin F synthase gene delivery in monkey eyes in vivo.
Lee, Eun Suk; Rasmussen, Carol A; Filla, Mark S; Slauson, Sarah R; Kolb, Aaron W; Peters, Donna M; Kaufman, Paul L; Gabelt, B'Ann T; Brandt, Curtis R.
Afiliación
  • Lee ES; Department of Ophthalmology, Institute of Vision Research, Yonsei University College of Medicine , Seoul , Korea .
Curr Eye Res ; 39(9): 859-70, 2014 Sep.
Article en En | MEDLINE | ID: mdl-24559478
Currently, the most effective outflow drugs approved for clinical use are prostaglandin F2α analogues, but these require daily topical self-dosing and have various intraocular, ocular surface and extraocular side effects. Lentiviral vector-mediated delivery of the prostaglandin F synthase (PGFS) gene, resulting in long-term reduction of intraocular pressure (IOP), may eliminate off-target tissue effects and the need for daily topical PGF2α self-administration. Lentiviral vector-mediated delivery of the PGFS gene to the anterior segment has been achieved in cats and non-human primates. Although these results are encouraging, our studies have identified a number of challenges that need to be overcome for prostaglandin gene therapy to be translated into the clinic. Using examples from our work in non-human primates, where we were able to achieve a significant reduction in IOP (2 mm Hg) for 5 months after delivery of the cDNA for bovine PGF synthase, we identify and discuss these issues and consider several possible solutions.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hidroxiprostaglandina Deshidrogenasas / Técnicas de Transferencia de Gen / Lentivirus / Vectores Genéticos Límite: Animals Idioma: En Revista: Curr Eye Res Año: 2014 Tipo del documento: Article Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hidroxiprostaglandina Deshidrogenasas / Técnicas de Transferencia de Gen / Lentivirus / Vectores Genéticos Límite: Animals Idioma: En Revista: Curr Eye Res Año: 2014 Tipo del documento: Article Pais de publicación: Reino Unido