GPR40 activation leads to CREB and ERK phosphorylation in primary cultures of neurons from the mouse CNS and in human neuroblastoma cells.
Hippocampus
; 24(7): 733-9, 2014 Jul.
Article
en En
| MEDLINE
| ID: mdl-24550142
GPR40, the free fatty acid receptor 1, is expressed strongly in the primate pancreas and brain. While the role of pancreatic GPR40 in glucose homeostasis has been extensively studied, the absence of this G-protein-coupled receptor from the brain of rodents has hampered studies into its role in the central nervous system. However, we found intense GPR40 mRNA expression by in situ hybridization in mouse hippocampal and motor cortex neurons. Furthermore, in a neuroblastoma cell GPR40 was activated by docosahexaenoic acid and selective agonists, yet not by palmitic acid. Significantly, the activation of GPR40 provoked the phosphorylation of the cAMP response element-binding protein, CREB. The receptor was also functional in primary cultures of murine neurons, in which its activation by a selective agonist produced the phosphorylation of CREB and of extracellular signal-regulated kinases, ERK1/2. These results suggest that mice represent a suitable model for elucidating the role of GPR40 in brain function.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteína de Unión a Elemento de Respuesta al AMP Cíclico
/
Receptores Acoplados a Proteínas G
/
Proteínas del Tejido Nervioso
/
Neuronas
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Hippocampus
Asunto de la revista:
CEREBRO
Año:
2014
Tipo del documento:
Article
País de afiliación:
España
Pais de publicación:
Estados Unidos