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Antibody validation of immunohistochemistry for biomarker discovery: recommendations of a consortium of academic and pharmaceutical based histopathology researchers.
Howat, William J; Lewis, Arthur; Jones, Phillipa; Kampf, Caroline; Pontén, Fredrik; van der Loos, Chris M; Gray, Neil; Womack, Chris; Warford, Anthony.
Afiliación
  • Howat WJ; Cancer Research UK Cambridge Institute, University of Cambridge, Li Ka Shing Centre, Cambridge CB2 0RE, UK. Electronic address: will.howat@cruk.cam.ac.uk.
  • Lewis A; MedImmune, Granta Park, Cambridge, UK.
  • Jones P; UCL Advanced Diagnostics, 21 University Street, London WC1E 6JJ, UK.
  • Kampf C; Department of Immunology, Genetics and Pathology, Rudbeck Laboratory, Uppsala University, Uppsala, Sweden.
  • Pontén F; Department of Immunology, Genetics and Pathology, Rudbeck Laboratory, Uppsala University, Uppsala, Sweden.
  • van der Loos CM; Department of Pathology, Academic Medical Center, Amsterdam, The Netherlands.
  • Gray N; AstraZeneca Oncology Innovative Medicines, Macclesfield, UK.
  • Womack C; AstraZeneca Oncology Innovative Medicines, Macclesfield, UK.
  • Warford A; University of Westminster, London, UK.
Methods ; 70(1): 34-8, 2014 Nov.
Article en En | MEDLINE | ID: mdl-24525140
As biomarker discovery takes centre-stage, the role of immunohistochemistry within that process is increasing. At the same time, the number of antibodies being produced for "research use" continues to rise and it is important that antibodies to be used as biomarkers are validated for specificity and sensitivity before use. This guideline seeks to provide a stepwise approach for the validation of an antibody for immunohistochemical assays, reflecting the views of a consortium of academic and pharmaceutical based histopathology researchers. We propose that antibodies are placed into a tier system, level 1-3, based on evidence of their usage in immunohistochemistry, and that the degree of validation required is proportionate to their place on that tier.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Inmunohistoquímica / Biomarcadores / Proteínas / Anticuerpos Tipo de estudio: Guideline Límite: Animals / Humans Idioma: En Revista: Methods Asunto de la revista: BIOQUIMICA Año: 2014 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Inmunohistoquímica / Biomarcadores / Proteínas / Anticuerpos Tipo de estudio: Guideline Límite: Animals / Humans Idioma: En Revista: Methods Asunto de la revista: BIOQUIMICA Año: 2014 Tipo del documento: Article Pais de publicación: Estados Unidos